Files and links (1)
Published, Version of Record (VoR)Open Access (License Unspecified), Open
Journal article
Juvenile and mature MAP2 isoforms induce distinct patterns of process outgrowth
Molecular biology of the cell, v 7(3), pp 443-455
Mar 1996
PMID: 8868472
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Microtubule-associated protein-2 (MAP2) is the most abundant MAP in neurons, where its distribution is restricted to the somatodendritic compartment. This molecule undergoes developmentally regulated alternative splicing, resulting in at least two isoforms, a juvenile isoform (termed MAP2c) and a mature isoform (MAP2), with greatly different molecular masses. Spodoptera frugiperda (Sf9) cell expression of the juvenile versus the mature MAP2 isoform generates two distinct patterns of process outgrowth. The smaller juvenile isoform induces multiple short thin processes. Mature MAP2 tends to induce single processes that are considerably thicker than those processes induced by juvenile MAP2. We found important differences in the variability of spacing between microtubules and the number of microtubules along the processes induced by MAP2c and mature MAP2. MAP2c showed variability with most microtubules spaced as closely as with tau, but some spaced as far apart as with mature MAP2. Over their length, the mature MAP2 processes demonstrate proximo-distal taper, which corresponds to a narrowing of the spacing between microtubules from 90 nm to 40 nm. Moreover, there is a decreased number of microtubules in mature MAP2-induced processes whereas in tau and MAP2-induced processes, the number of microtubules is constant along the length. Based on these observations, we conclude that MAP2 isoforms can serve as architectural elements by establishing specific morphological features of processes and specific arrangements of their microtubules.
Metrics
Details
- Title
- Juvenile and mature MAP2 isoforms induce distinct patterns of process outgrowth
- Creators
- N Leclerc - Département de Pathologie, Université de Montréal, Québec, CanadaP W Baas - Département de Pathologie, Université de Montréal, Québec, CanadaC C Garner - Département de Pathologie, Université de Montréal, Québec, CanadaK S Kosik - Département de Pathologie, Université de Montréal, Québec, Canada
- Publication Details
- Molecular biology of the cell, v 7(3), pp 443-455
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1996TZ76100009
- Scopus ID
- 2-s2.0-0029921189
- Other Identifier
- 991014878593804721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology