Journal article
Katanin-p80 Gene Promoter Characterization and Regulation via Elk1
PloS one, v 8(7), 69423
24 Jul 2013
PMID: 23894477
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Katanin is an ATPase family member protein that participates in microtubule severing. It has heterodimeric structure consisting of 60 kDa (katanin-p60) and 80 kDa (katanin-p80) subunits encoded by KATNA1 and KATNB1 genes, respectively. Katanin-p60 has the enzymatic activity for microtubule severing, whereas katanin-p80 consists of multiple domains with different functions such as targeting katanin-p60 to the centrosome, augmenting microtubule severing by katanin-p60, and even suppressing microtubule severing. Despite the various important functions of katanin-p80, its transcriptional regulation has not been studied yet. Elk1 transcription factor has been shown to interact with microtubules and regulate the transcription of another microtubule severing protein, spastin. In spite of katanin's importance, and structural and functional similarities to spastin, there is no study on the transcriptional regulation of katanin yet. In this study, we aimed to characterize KATNB1 promoter and analyze the effects of Elk1 on katanin-p80 expression. We identified a 518-bp TATA-less promoter including a critical CpG island and GC boxes as an optimal promoter, and sequential deletion of CpG island and the GC elements gradually decreased the KATNB1 promoter activity. In addition, we showed Elk1 binding on the KATNB1 promoter by EMSA. We found that Elk1 activated KATNB1 promoter, and increased both mRNA and protein levels of katanin-p80 in SH-SY5Y cells. On the other hand, KCl treatment increasing SUMOylation decreased KATNB1 promoter activity. Since microtubule severing is an important cellular mechanism of which malfunctions result in serious diseases such as spastic paraplegia, Alzheimer's disease and cell cycle related disorders, identification of KATNB1 transcriptional regulation is crucial in understanding the coordination of microtubule severing activity by different proteins in the cells.
Metrics
4 Record Views
Details
- Title
- Katanin-p80 Gene Promoter Characterization and Regulation via Elk1
- Creators
- Ece Selcuk - Istanbul Technical UniversityKoray Kirimtay - Istanbul Technical UniversityDerya Canbaz - Istanbul Technical UniversityGuher Isik Cesur - Istanbul Technical UniversitySirin Korulu - Istanbul Technical UniversityArzu Karabay - Istanbul Technical University
- Publication Details
- PloS one, v 8(7), 69423
- Publisher
- Public Library Science
- Number of pages
- 13
- Grant note
- 34776 / Istanbul Technical University, Scientific Research Projects Department; Istanbul Technical University
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000322167900068
- Scopus ID
- 2-s2.0-84880840894
- Other Identifier
- 991022018186704721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology