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Keratin Durability Has Implications for the Fossil Record: Results from a 10 Year Feather Degradation Experiment
Journal article   Open access   Peer reviewed

Keratin Durability Has Implications for the Fossil Record: Results from a 10 Year Feather Degradation Experiment

Alison E Moyer, Wenxia Zheng and Mary H Schweitzer
PloS one, v 11(7), pp e0157699-e0157699
06 Jul 2016
PMID: 27384819
url
https://doi.org/10.1371/journal.pone.0157699View
Published, Version of Record (VoR) Open

Abstract

Animals Antibodies - chemistry Biological Evolution Chickens Epidermis - anatomy & histology Feathers - chemistry Fossils Humans Keratins - chemistry Microscopy, Electron, Transmission Microscopy, Fluorescence Nails Phylogeny Temperature
Keratinous 'soft tissue' structures (i.e. epidermally derived and originally non-biomineralized), include feathers, skin, claws, beaks, and hair. Despite their relatively common occurrence in the fossil record (second only to bone and teeth), few studies have addressed natural degradation processes that must occur in all organic material, including those keratinous structures that are incorporated into the rock record as fossils. Because feathers have high preservation potential and strong phylogenetic signal, in the current study we examine feathers subjected to different burial environments for a duration of ~10 years, using transmission electron microscopy (TEM) and in situ immunofluorescence (IF). We use morphology and persistence of specific immunoreactivity as indicators of preservation at the molecular and microstructural levels. We show that feather keratin is durable, demonstrates structural and microstructural integrity, and retains epitopes suitable for specific antibody recognition in even the harshest conditions. These data support the hypothesis that keratin antibody reactivity can be used to identify the nature and composition of epidermal structures in the rock record, and to address evolutionary questions by distinguishing between alpha- (widely distributed) and beta- (limited to sauropsids) keratin.

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Web of Science research areas
Evolutionary Biology
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