Journal article
Kinesin-12 influences axonal growth during zebrafish neural development
Cytoskeleton (Hoboken, N.J.), v 71(10), pp 555-563
Oct 2014
PMID: 25250533
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Kinesin-12 (also called KIF15) is a microtubule-based motor protein best known for its role in cell division. We previously reported that kinesin-12 is robustly expressed in developing terminally post-mitotic neurons, with levels diminishing as neurons reach maturity. We found that axons of cultured rodent neurons grow faster and longer if kinesin-12 is experimentally depleted, leading us to conclude that kinesin-12 plays a role in modulating axonal growth. Here we used zebrafish to explore whether these results apply to an
in vivo
system and whether they apply across different kinds of vertebrates. In whole mount
in situ
hybridization, kinesin-12 mRNA was detectable at 2-cell and 1K-cell stages. At 5.3 and 8 hours post-fertilization (hpf), hybridization signal for kinesin-12 mRNA was observed in the ectoderm. From 14 to 36 hpf, the signal had expanded to the central nervous system. At 60 hpf, the hybridization signal was concentrated in the brain. After 5 days post-fertilization, kinesin-12 expression was reduced. Kinesin-12 knockdown resulted in notably longer fast-growing axons with fewer branches by injection of a splice-blocking morpholino into
Tg(huC:egfp)
or
Tg(hb9:gfp)
zebrafish embryos. Kinesin-12 overexpression resulted in shorter axons than controls. These results are consistent with our previous observations on rodents using primary cultures for the experimental manipulations, and suggest a key role of kinesin-12 as a modulator of axonal development.
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Details
- Title
- Kinesin-12 influences axonal growth during zebrafish neural development
- Creators
- Man Xu - Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong Jiangsu 226001, ChinaDong Liu - Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong Jiangsu 226001, ChinaZhangji Dong - Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong Jiangsu 226001, ChinaXin Wang - Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong Jiangsu 226001, ChinaXueqian Wang - Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong Jiangsu 226001, ChinaYan Liu - Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong Jiangsu 226001, ChinaPeter W Baas - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA, USAMei Liu - Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong Jiangsu 226001, China
- Publication Details
- Cytoskeleton (Hoboken, N.J.), v 71(10), pp 555-563
- Publisher
- Wiley
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000345273100001
- Scopus ID
- 2-s2.0-84910017002
- Other Identifier
- 991014877654904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology