Journal article
Kinesin-5 Is Essential for Growth-Cone Turning
Current biology, v 18(24), pp 1972-1977
23 Dec 2008
PMID: 19084405
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Inhibition of kinesin-5, a mitotic motor protein also expressed in neurons [1], causes axons to grow faster as a result of alterations in the forces on microtubules (MTs) in the axonal shaft [2–4]. Here, we investigate whether kinesin-5 plays a role in growth-cone guidance. Growth-cone turning requires that MTs in the central (C-) domain enter the peripheral (P-) domain in the direction of the turn. We found that inhibition of kinesin-5 in cultured neurons prevents MTs from polarizing within growth cones and causes them to grow past cues that would normally cause them to turn. We found that kinesin-5 is enriched in the transition (T-) zone of the growth cone and that kinesin-5 is preferentially phosphorylated on the side opposite the invasion of MTs. Moreover, when a growth cone encounters a turning cue, phospho-kinesin-5 polarizes even before the growth cone turns. Additional studies indicate that kinesin-5 works in part by antagonizing cytoplasmic dynein and that these motor-driven forces function together with the dynamic properties of the MTs to determine whether MTs can enter the P-domain. We propose that kinesin-5 permits MTs to selectively invade one side of the growth cone by opposing their entry into the other side.
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Details
- Title
- Kinesin-5 Is Essential for Growth-Cone Turning
- Creators
- Vidya C Nadar - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USAAndrea Ketschek - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USAKenneth A Myers - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USAGianluca Gallo - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USAPeter W Baas - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA
- Publication Details
- Current biology, v 18(24), pp 1972-1977
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000262089700035
- Scopus ID
- 2-s2.0-57649188646
- Other Identifier
- 991014877789704721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biology
- Cell Biology