Journal article
Kinesin-5 regulates the growth of the axon by acting as a brake on its microtubule array
The Journal of cell biology, v 178(6), pp 1081-1091
10 Sep 2007
PMID: 17846176
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Kinesin-5 is a homotetrameric motor protein that interacts with adjacent microtubules in the mitotic spindle. Kinesin-5 is also highly expressed in developing postmitotic neurons. Axons of cultured neurons experimentally depleted of kinesin-5 grow up to five times longer than controls and display more branches. The faster growth rates are accompanied by a doubling of the frequency of transport of short microtubules, suggesting a major role for kinesin-5 in the balance of motor-driven forces on the axonal microtubule array. Live-cell imaging reveals that the effects on axonal length of kinesin-5 depletion are caused partly by a lower propensity of the axon and newly forming branches to undergo bouts of retraction. Overexpression of wild-type kinesin-5, but not a rigor mutant of kinesin-5, has the inverse effect on axonal length. These results indicate that kinesin-5 imposes restrictions on the growth of the axon and does so at least in part by generating forces on the axonal microtubule array.
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Details
- Title
- Kinesin-5 regulates the growth of the axon by acting as a brake on its microtubule array
- Creators
- Kenneth A Myers - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19104Peter W Baas - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19104
- Publication Details
- The Journal of cell biology, v 178(6), pp 1081-1091
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000249498900016
- Scopus ID
- 2-s2.0-34548833602
- Other Identifier
- 991014878300404721
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- Web of Science research areas
- Cell Biology