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Kinetic studies with sarcosine, alternate substrates, and a substrate analogue
Journal article   Peer reviewed

Kinetic studies with sarcosine, alternate substrates, and a substrate analogue

Mary Ann Wagner and Marilyn Schuman Jorns
Biochemistry (Easton), Vol.39(30), p8825
01 Aug 2000

Abstract

Amines Catalysis Charge transfer Cytochemistry Microbial enzymes Oxidases Physiological aspects
Kinetic studies of monomeric sarcosine oxidase (MSOX), a flavoenzyme that catalyzes the oxidative demethylation of sarcosine, N-methylglycine) have been carried out using alternate substrates, sarcosine, and a substrate analogue. MSOX does not form a 4a-adduct with thioglycolate. It does form a charge-transfer complex that goes through an unexpected one-electron-transfer reaction to give the anionic flavin radical.

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