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Kinetics and localization of interleukin-2, interleukin-6, heat shock protein 70, and interferon gamma during intestinal-rerfusion injury
Journal article   Peer reviewed

Kinetics and localization of interleukin-2, interleukin-6, heat shock protein 70, and interferon gamma during intestinal-rerfusion injury

F Braun, M Hosseini, E Wieland, B Sattler, A.R Müller, F Fändrich, B Kremer and B Ringe
Transplantation proceedings, v 36(2), pp 267-269
2004
DOI: https://doi.org/10.1016/j.transproceed.2004.01.082
PMID: 15050130
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Intestinal ischemia-reperfusion injury (IRI) represents an exaggerated inflammatory cascade with a complex pathophysiology. IL-2, IL-6, HSP70, and INF-γ are mediators of the inflammatory process. Therefore, we investigated their kinetics and localization during intestinal IRI. Pig intestinal specimens were obtained during cold preservation (cold ischemia time 2 hours) and extracorporeal perfusion. Mucosal damage was graded according to the Chiu classification. MRNA expression was determined by Northern blot (IL-2, IL-6, IFN-γ) or by quantitative RT-PCR (IL-6, HSP70) and localized by in situ hybridization. Histologically, mucosal damge occurred during reperfusion. Expression of IL-2 mRNA was up-regulated after HTK perfusion and was highest at the start and 7 hours after reperfusion. Expression of IL-6 mRNA increased at 2 hours after reperfusion and HSP70 at 3 hours after reperfusion. IFN-γ mRNA was expressed after HTK perfusion, with expression of this cytokine increasing to 1 hour after the start of reperfusion, and decreasing thereafter. IL-2 mRNA was localized to endothelial cells (EC) and leukocytes and in close relation to ganglion cells (GC): IL-6 mRNA in EC, smooth muscle cells (SMC), and GC: HSP70 mRNA in EC and SMC; and IFN-γ mRNA in leukocytes. IL-2, IL-6, HSP70, and INF-γ are parameters of early mRNA expression during intestinal IRI. EC, SMC, leukocytes, and GC have been identified as sources of transcripts that might afford potential targets for intervention strategies to attenuate IRI.

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Domestic collaboration
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Web of Science research areas
Immunology
Surgery
Transplantation
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