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Kismet positively regulates glutamate receptor localization and synaptic transmission at the Drosophila neuromuscular junction
Journal article   Open access

Kismet positively regulates glutamate receptor localization and synaptic transmission at the Drosophila neuromuscular junction

Rupa Ghosh, Srikar Vegesna, Ramia Safi, Hong Bao, Bing Zhang, Daniel R Marenda and Faith L W Liebl
PloS one, v 9(11), pp e113494-e113494
2014
PMID: 25412171
url
https://doi.org/10.1371/journal.pone.0113494View
Published, Version of Record (VoR) Open

Abstract

Immunohistochemistry Receptors, Glutamate - metabolism Synaptic Transmission - physiology Neuromuscular Junction - metabolism Homeodomain Proteins - metabolism Larva - metabolism Chromatin Assembly and Disassembly Synapses - pathology Drosophila Proteins - metabolism DNA-Binding Proteins - metabolism Motor Neurons - metabolism DNA Helicases - metabolism Animals Cell Nucleus - metabolism Synapses - metabolism Drosophila - growth & development Drosophila - metabolism Drosophila Proteins - genetics Locomotion - physiology Muscles - metabolism
The Drosophila neuromuscular junction (NMJ) is a glutamatergic synapse that is structurally and functionally similar to mammalian glutamatergic synapses. These synapses can, as a result of changes in activity, alter the strength of their connections via processes that require chromatin remodeling and changes in gene expression. The chromodomain helicase DNA binding (CHD) protein, Kismet (Kis), is expressed in both motor neuron nuclei and postsynaptic muscle nuclei of the Drosophila larvae. Here, we show that Kis is important for motor neuron synaptic morphology, the localization and clustering of postsynaptic glutamate receptors, larval motor behavior, and synaptic transmission. Our data suggest that Kis is part of the machinery that modulates the development and function of the NMJ. Kis is the homolog to human CHD7, which is mutated in CHARGE syndrome. Thus, our data suggest novel avenues of investigation for synaptic defects associated with CHARGE syndrome.

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Collaboration types
Domestic collaboration
Web of Science research areas
Neurosciences
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