Journal article
Ku80 facilitates chromatin binding of the telomere binding protein, TRF2
Cell cycle (Georgetown, Tex.), v 9(18), pp 3798-3806
15 Sep 2010
PMID: 20890109
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The Ku70/80 heterodimer is central to non-homologous end joining repair of DNA double-strand breaks and the Ku80 gene appears to be essential for human but not rodent cell survival. The Ku70/80 heterodimer is located at telomeres but its precise function in telomere maintenance is not known. In order to examine the role of Ku80 beyond DNA repair in more detail, we have taken a knockdown approach using a human fibroblast strain. Following targeted Ku80 knockdown, telomere defects are observed and the steady state levels of the TRF2 protein are reduced. Inhibitor studies indicate that this loss of TRF2 is mediated by the proteasome and degradation of TRF2 following Ku depletion appears to involve a decrease in chromatin binding of TRF2, suggesting that the Ku heterodimer enhances TRF2 chromatin association and that non-chromatin bound TRF2 is targeted to the proteasome.
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Details
- Title
- Ku80 facilitates chromatin binding of the telomere binding protein, TRF2
- Creators
- Lauren S Fink - Drexel UniversityChad A LernerPaulina F TorresChristian Sell - Drexel University
- Publication Details
- Cell cycle (Georgetown, Tex.), v 9(18), pp 3798-3806
- Publisher
- Landes Bioscience
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000281909900030
- Scopus ID
- 2-s2.0-77957002731
- Other Identifier
- 991019169554704721
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- Web of Science research areas
- Cell Biology