Journal article
Kv3.3b: a novel Shaw type potassium channel expressed in terminally differentiated cerebellar Purkinje cells and deep cerebellar nuclei
The Journal of neuroscience, v 14(2), pp 511-522
01 Feb 1994
PMID: 8301351
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A two-step hybridization/subtraction procedure was employed to isolate markers for the later stages of Purkinje cell differentiation. From this screen, a novel Shaw potassium channel cDNA (Kv3.3b) was identified that is developmentally regulated. Expression of this channel is highly enriched in the brain, particularly in the cerebellum, where its expression is confined to Purkinje cells and deep cerebellar nuclei. Sequence analysis revealed that it is an alternatively spliced form of the mouse Kv3.3 gene, and that the previously reported Kv3.3 mRNA (Ghanshani et al., 1992) is not expressed in cerebellum. Expression of the Kv3.3b mRNA begins in cerebellar Purkinje cells between postnatal day 8 (P8) and P10 and continues through adulthood, coinciding with elaboration of the mature Purkinje cell dendritic arbor. The timing of expression of Kv3.3b mRNA is maintained in mixed, dissociated primary cerebellar cell culture. These results suggest that the Kv3.3b K+ channel function is restricted to terminally differentiated Purkinje cells, and that analysis of the mechanisms governing its expression in vivo and in vitro can reveal molecular mechanisms governing Purkinje cell differentiation.
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Details
- Title
- Kv3.3b: a novel Shaw type potassium channel expressed in terminally differentiated cerebellar Purkinje cells and deep cerebellar nuclei
- Creators
- D S Goldman-Wohl - Howard Hughes Medical InstituteE ChanD BairdN Heintz
- Publication Details
- The Journal of neuroscience, v 14(2), pp 511-522
- Publisher
- Society for Neuroscience
- Grant note
- GM07739-14 / NIGMS NIH HHS NS08815-02 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Intensive Medical Sciences (IMS)
- Web of Science ID
- WOS:A1994MV31900005
- Scopus ID
- 2-s2.0-0028107480
- Other Identifier
- 991020202229404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences