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LAN-1 human neuroblastoma cells are provided of endothelin-1 receptors linked to [Ca++]i elevation
Journal article   Peer reviewed

LAN-1 human neuroblastoma cells are provided of endothelin-1 receptors linked to [Ca++]i elevation

A Fatatis, A Bassi, G Mita, G F Di Renzo and L Annunziato
Functional neurology, v 7(3), pp 239-242
May 1992
PMID: 1323509

Abstract

Cell Line Endothelins - pharmacology Calcium - metabolism Humans Tumor Cells, Cultured - drug effects Intracellular Fluid - physiology Neuroblastoma Dose-Response Relationship, Drug Intracellular Fluid - drug effects Receptors, Endothelin Receptors, Cell Surface - drug effects Receptors, Cell Surface - physiology Microscopy, Fluorescence
Endothelin-1 (ET-1) produced a dose-dependent increase of intracellular Ca++ concentrations [Ca++]i characterized by an early peak phase and a delayed plateau in LAN-1 human neuroblastoma cells. The ET-1 receptor showed a rapid desensitization since a second pulse application of ET-1 did not elicit a further [Ca++]i increase. Furthermore thapsigargin, an endoplasmic reticulum Ca(++)-ATPase inhibitor, completely abolished the ET-1 induced intracellular Ca++ elevation.

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