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Journal article
LC-MS based stability-indicating method for studying the degradation of lonidamine under physical and chemical stress conditions
Research in pharmaceutical sciences, v 15(4), pp 312-322
01 Jul 2020
PMID: 33312209
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background and purpose: Lonidamine is a hexokinase II inhibitor, works as an anticancer molecule, and is extensively explored in clinical trials. Limited information prevails about the stability-indicating methods which could determine the forced degradation of lonidamine under stressed conditions. Hence, we report the use of a rapid, sensitive, reproducible, and highly accurate liquid chromatography and mass spectrometry method to analyze lonidamine degradation.
Experimental approach: The Xbridge BEH shield reverse phase C18 column (2.5 mu m, 4.6 x 75 mm) using isocratic 50:50 water: acetonitrile with 0.1% formic acid can detect lonidamine with help of mass spectrometer in tandem with an ultraviolet (UV) detector at 260 nm wavelength.
Findings/ Results: A linear curve with r(2) > 0.99 was obtained for tandem liquid chromatography-mass spectrometry (LC-MS)-UV based detections. This study demonstrated (in the present set up of isocratic elution) that LC-MS based detection has a relatively high sensitivity (S/N (10 ng/mL): 220 and S/N (20 ng/mL): 945) and accuracy at lower detection and quantitation levels, respectively. In addition to developing the LC-MS method, we also report that the current method is stability-indicating and shows that lonidamine gets degraded over time under all three stress conditions; acidic, basic, and oxidative.
Conclusion and implications: LC-MS based quantitation of lonidamine proved to be a better method compared to high-performance liquid chromatography (HPLC)-UV detections for mapping lonidamine degradation. This is the first report on the stability-indicating method for studying the forced degradation of lonidamine using LC-MS method.
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Details
- Title
- LC-MS based stability-indicating method for studying the degradation of lonidamine under physical and chemical stress conditions
- Creators
- Ankit Kanaiyalal Rochani - Thomas Jefferson UniversityMargaret Wheatley - Drexel UniversityBrian Edward Oeffinger - Drexel UniversityJohn Robert Eisenbrey - Thomas Jefferson UniversityGagan Kaushal - Thomas Jefferson University
- Publication Details
- Research in pharmaceutical sciences, v 15(4), pp 312-322
- Publisher
- Wolters Kluwer Medknow Publications
- Number of pages
- 11
- Grant note
- R01EB023926 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000570938800001
- Scopus ID
- 2-s2.0-85091069481
- Other Identifier
- 991019168859004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Medicinal