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LEVER: software tools for segmentation, tracking and lineaging of proliferating cells
Journal article   Open access   Peer reviewed

LEVER: software tools for segmentation, tracking and lineaging of proliferating cells

Mark Winter, Walter Mankowski, Eric Wait, Sally Temple and Andrew R. Cohen
BIOINFORMATICS, v 32(22), pp 3530-3531
15 Nov 2016
PMID: 27423896
url
https://doi.org/10.1093/bioinformatics/btw406View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze) Open

Abstract

Biochemical Research Methods Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Computer Science Computer Science, Interdisciplinary Applications Life Sciences & Biomedicine Mathematical & Computational Biology Mathematics Physical Sciences Science & Technology Statistics & Probability Technology
The analysis of time-lapse images showing cells dividing to produce clones of related cells is an important application in biological microscopy. Imaging at the temporal resolution required to establish accurate tracking for vertebrate stem or cancer cells often requires the use of transmitted light or phase-contrast microscopy. Processing these images requires automated segmentation, tracking and lineaging algorithms. There is also a need for any errors in the automated processing to be easily identified and quickly corrected. We have developed LEVER, an open source software tool that combines the automated image analysis for phase-contrast microscopy movies with an easy-to-use interface for validating the results and correcting any errors.

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39 citations in Scopus

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Web of Science research areas
Biochemical Research Methods
Biotechnology & Applied Microbiology
Computer Science, Interdisciplinary Applications
Mathematical & Computational Biology
Statistics & Probability
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