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LFM-Pro: a tool for detecting significant local structural sites in proteins
Journal article   Open access   Peer reviewed

LFM-Pro: a tool for detecting significant local structural sites in proteins

Ahmet Sacan, Ozgur Ozturk, Hakan Ferhatosmanoglu and Yusu Wang
Bioinformatics (Oxford, England), v 23(6), pp 709-716
15 Mar 2007
PMID: 17237050
url
https://doi.org/10.1093/bioinformatics/btl685View
Published, Version of Record (VoR) Open

Abstract

Motivation: The rapidly growing protein structure repositories have opened up new opportunities for discovery and analysis of functional and evolutionary relationships among proteins. Detecting conserved structural sites that are unique to a protein family is of great value in identification of functionally important atoms and residues. Currently available methods are computationally expensive and fail to detect biologically significant local features. Results: We propose Local Feature Mining in Proteins (LFM-Pro) as a framework for automatically discovering family-specific local sites and the features associated with these sites. Our method uses the distance field to backbone atoms to detect geometrically significant structural centers of the protein. A feature vector is generated from the geometrical and biochemical environment around these centers. These features are then scored using a statistical measure, for their ability to distinguish a family of proteins from a background set of unrelated proteins, and successful features are combined into a representative set for the protein family. The utility and success of LFM-Pro are demonstrated on trypsin-like serine proteases family of proteins and on a challenging classification dataset via comparison with DALI. The results verify that our method is successful both in identifying the distinctive sites of a given family of proteins, and in classifying proteins using the extracted features. Availability: The software and the datasets are freely available for academic research use at http://bioinfo.ceng.metu.edu.tr/Pub/LFMPro Contact: ahmet@ceng.metu.edu.tr, ozturk@cse.ohiostate.edu,hakan@cse.ohiostate.edu,yusu@cse.ohiostate.edu

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Biochemical Research Methods
Biotechnology & Applied Microbiology
Computer Science, Interdisciplinary Applications
Mathematical & Computational Biology
Statistics & Probability
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