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Lab-on-a-chip technologies for oral-based cancer screening and diagnostics: capabilities, issues, and prospects
Journal article

Lab-on-a-chip technologies for oral-based cancer screening and diagnostics: capabilities, issues, and prospects

Michael G Mauk, Barry L Ziober, Zongyuan Chen, Jason A Thompson and Haim H Bau
Annals of the New York Academy of Sciences, v 1098(1), pp 467-475
Mar 2007
PMID: 17435153

Abstract

Predictive Value of Tests Antibodies, Neoplasm - analysis Antigens, Neoplasm - immunology Carcinoma, Squamous Cell - pathology Humans Carcinoma, Squamous Cell - chemistry Lab-On-A-Chip Devices Mouth Neoplasms - chemistry Feasibility Studies Carcinoma, Squamous Cell - diagnosis Mouth Neoplasms - diagnosis Mass Screening Mouth Neoplasms - pathology Microchip Analytical Procedures - economics Microfluidics
The design of a microfluidic lab-on-a-chip system for point-of-care cancer screening and diagnosis of oral squamous cell carcinoma (OSCC) is presented. The chip is based on determining a approximately 30-gene transcription profile in cancer cells isolated from oral fluid samples. Microfluidic cell sorting using magnetic beads functionalized with an antibody against cancer-specific cell-surface antigens (e.g., epithelial cell adhesion molecule [EpCAM]) is described. A comprehensive cancer diagnostics chip will integrate microfluidic components for cell lysis, nucleic acid extraction, and amplification and detection of a panel of mRNA isolated from a subpopulation of cancer cells contained in a clinical specimen.

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Web of Science research areas
Dentistry, Oral Surgery & Medicine
Medical Laboratory Technology
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