Journal article
Label-free Her2 detection and dissociation constant assessment in diluted human serum using a longitudinal extension mode of a piezoelectric microcantilever sensor
Sensors and actuators. B, Chemical, v 160(1), pp 349-356
2011
PMID: 22888196
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have investigated real-time, label-free, in situ detection of human epidermal growth factor receptor 2 (Her2) in diluted serum using the first longitudinal extension mode of a lead zirconate-lead titanate (PZT)/glass piezoelectric microcantilever sensor (PEMS) with H3 single-chain variable fragment (scFv) immobilized on the 3-mercaptopropyltrimethoxysilane (MPS) insulation layer of the PEMS surface. We showed that with the longitudinal extension mode, the PZT/glass PEMS consisting of a 1
mm long and 127
μm thick PZT layer bonded with a 75
μm thick glass layer with a 1.8
mm long glass tip could detect Her2 at a concentration of 6–60
ng/ml (or 0.06–0.6
nM) in diluted human serum, about 100 times lower than the concentration limit obtained using the lower-frequency flexural mode of a similar PZT/glass PEMS. We further showed that with the longitudinal mode, the PZT/glass PEMS determined the equilibrium H3–Her2 dissociation constant
K
d to be 3.3
±
0.3
×
10
−8
M consistent with the value, 3.2
±
0.28
×
10
−8
M deduced by the surface plasmon resonance method (BIAcore).
Metrics
Details
- Title
- Label-free Her2 detection and dissociation constant assessment in diluted human serum using a longitudinal extension mode of a piezoelectric microcantilever sensor
- Creators
- Joseph A Capobianco - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, United StatesWan Y Shih - School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, PA 19104, United StatesGregory P Adams - Developmental Therapeutics Program, Fox Chase Cancer Center, Philadelphia, PA, United StatesWei-Heng Shih - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, United States
- Publication Details
- Sensors and actuators. B, Chemical, v 160(1), pp 349-356
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering
- Web of Science ID
- WOS:000298768100050
- Scopus ID
- 2-s2.0-81155149870
- Other Identifier
- 991014877875204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical
- Electrochemistry
- Instruments & Instrumentation