Journal article
Label-free, all-electrical, in situ human epidermal growth receptor 2 detection
Review of scientific instruments, v 79(7), pp 076101-076101
Jul 2008
PMID: 18681738
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Using 3-mercaptopropyltrimethoxysilane (MPS)-coated (PbMg 1/3 Nb 2/3 O3)0.63-(PbTiO3)0.37 (PMN-PT)/tin and lead zirconate titanate/glass piezoelectric microcantilever sensors (PEMSs) with single-chain variable fragment (scFv) immobilized on the MPS surface, we have demonstrated real-time, label-free detection of human epidermal growth factor receptor 2 (Her2) in a background of 1 mg/ml bovine serum albumin. Coupled with a scFv with a KD of 3.4 x 10(-8)M, the MPS-insulated PMN-PT/tin PEMS 560 microm long and 720 microm wide exhibited a Her2 concentration sensitivity of 5 ng/ml in a background of 1 mg/ml BSA.
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Details
- Title
- Label-free, all-electrical, in situ human epidermal growth receptor 2 detection
- Creators
- Joseph A Capobianco - Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USAWan Y ShihQing-An YuanGregory P AdamsWei-Heng Shih
- Publication Details
- Review of scientific instruments, v 79(7), pp 076101-076101
- Publisher
- American Institute of Physics (AIP); United States
- Grant note
- R01 EB000720-01 / NIBIB NIH HHS R01 EB000720 / NIBIB NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering
- Web of Science ID
- WOS:000258179400055
- Scopus ID
- 2-s2.0-49149128682
- Other Identifier
- 991014878148704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Instruments & Instrumentation
- Physics, Applied