Journal article
Label free detection of white spot syndrome virus using lead magnesium niobate–lead titanate piezoelectric microcantilever sensors
Biosensors & bioelectronics, v 26(3), pp 964-969
2010
PMID: 20863681
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have investigated rapid, label free detection of white spot syndrome virus (WSSV) using the first longitudinal extension resonance peak of five lead–magnesium niobate–lead titanate (PMN–PT) piezoelectric microcantilever sensors (PEMS) 1050–700
μm long and 850–485
μm wide constructed from 8
μm thick PMN–PT freestanding films. The PMN–PT PEMS were encapsulated with a 3-mercaptopropyltrimethoxysilane (MPS) insulation layer and further coated with anti-VP28 and anti-VP664 antibodies to target the WSSV virions and nucleocapsids, respectively. By inserting the antibody coated PEMS in a flowing virion or nucleocapsid suspension, label free detection of the virions and nucleocapsids were respectively achieved by monitoring the PEMS resonance frequency shift. We showed that positive label free detection of both the virion and the nucleocapsid could be achieved at a concentration of 100
virions
(nucleocapsids)/ml or 10
virions
(nucleocapsids)/100
μl, comparable to the detection sensitivity of polymerase chain reaction (PCR). However, in contrast to PCR, PEMS detection was label free, in situ and rapid (less than 30
min), potentially requiring minimal or no sample preparation.
Metrics
Details
- Title
- Label free detection of white spot syndrome virus using lead magnesium niobate–lead titanate piezoelectric microcantilever sensors
- Creators
- Joseph A Capobianco - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, United StatesWei-Heng Shih - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, United StatesJiann-Horng Leu - College of Life Science, National Taiwan University, Taipei, TaiwanGrace Chu-Fang Lo - College of Life Science, National Taiwan University, Taipei, TaiwanWan Y Shih - School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, PA 19104, United States
- Publication Details
- Biosensors & bioelectronics, v 26(3), pp 964-969
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering
- Web of Science ID
- WOS:000283813600002
- Scopus ID
- 2-s2.0-77957330742
- Other Identifier
- 991014878274304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology