Journal article
Label-free nonenzymatic glycation monitoring of collagen scaffolds in type 2 diabetic mice by confocal Raman microspectroscopy
Journal of biomedical optics, v 20(2), pp 027002-027002
01 Feb 2015
PMID: 25671672
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Collagen is the key target of nonenzymatic glycation during physiopathological processes such as diabetes. The induced changes in the biochemical property of collagen by nonenzymatic glycation remain a major challenge to probe. This study investigated the use of confocal Raman microspectroscopy to label-free monitor the nonenzymatic glycation of collagen scaffolds from type 2 diabetic (T2D) mice at different timepoints (0, 4, 8, and 12 weeks). The glycated collagen scaffolds were obtained through the decellularized dermal matrix method to remove the epidermis layer, subcutaneous tissue, and cells in the dermis and to retain the collagen fibrils. Raman spectra showed no changes in Raman peak positions, which indicated that nonenzymatic glycation could produce no significant changes in the triple-helix structure of collagen in T2D mice. However, the relative intensity of the Raman bands at 921, 1033, 1244, 1274, 1346, 1635, and 1672 cm(-1) increased as diabetic time progressed. Correlation analysis suggested that the spectra of these bands had a high positive correlation with the expression of anti-advanced glycation end products obtained by immunofluorescence imaging of the same collagen scaffolds. Confocal Raman microspectroscopy proves a potential tool to label-free monitor the collagen changes caused by nonenzymatic glycation in T2D mice. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
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Details
- Title
- Label-free nonenzymatic glycation monitoring of collagen scaffolds in type 2 diabetic mice by confocal Raman microspectroscopy
- Creators
- Panpan Shi - S China Normal Univ, Coll Biophoton, 55 Zhongshan Ave West, Guangzhou 510631, Guangdong, Peoples R ChinaHanping Liu - S China Normal Univ, Coll Biophoton, 55 Zhongshan Ave West, Guangzhou 510631, Guangdong, Peoples R ChinaXiaoyuan Deng - S China Normal Univ, Coll Biophoton, 55 Zhongshan Ave West, Guangzhou 510631, Guangdong, Peoples R ChinaYing Jin - S China Normal Univ, Coll Biophoton, 55 Zhongshan Ave West, Guangzhou 510631, Guangdong, Peoples R ChinaQiannan Wang - S China Normal Univ, Coll Biophoton, 55 Zhongshan Ave West, Guangzhou 510631, Guangdong, Peoples R ChinaHao Liu - S China Normal Univ, Coll Biophoton, 55 Zhongshan Ave West, Guangzhou 510631, Guangdong, Peoples R ChinaMaosheng Chen - S China Normal Univ, Coll Biophoton, 55 Zhongshan Ave West, Guangzhou 510631, Guangdong, Peoples R ChinaXue Han - S China Normal Univ, Coll Biophoton, 55 Zhongshan Ave West, Guangzhou 510631, Guangdong, Peoples R China
- Publication Details
- Journal of biomedical optics, v 20(2), pp 027002-027002
- Publisher
- Spie-Soc Photo-Optical Instrumentation Engineers
- Number of pages
- 9
- Grant note
- College of Biophotonics of South China Normal University 81171379 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000350462900019
- Scopus ID
- 2-s2.0-84923165823
- Other Identifier
- 991020545413504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Optics
- Radiology, Nuclear Medicine & Medical Imaging