Journal article
Lactobacillus acidophilus (strain Scav) Postbiotic Metabolites Reduce Infection and Modulate Inflammation in an in vivo model of Pseudomonas aeruginosa wound infection
Journal of applied microbiology, v 136(3), lxaf061
Mar 2025
PMID: 40068933
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This study assessed the antibacterial, anti-biofilm, and immunomodulatory activity of Lactobacillus acidophilus (strain Scav) postbiotic (LaP) in a mouse model of Pseudomonas aeruginosa wound infection and evaluated the bioactive components of the LaP.
LaP was tested for P. aeruginosa clearance and immunomodulatory activity during wound infection. We show that LaP applied 1 hour after infection reduced tissue bacterial burden within 24 hours, and this reduction persisted for 5 days. Ciprofloxacin given once at the exact same time did not reduce bacteria load as compared to vehicle controls. LaP reduced plasma IL-6 and MCP-1 levels after 5 days. Wound tissue IL-6 and MCP-1 levels were increased in infected vehicle mice at 5 days, but tissues from LaP treated mice were similar to sham controls. LaP increased tissue IL-10 (anti-inflammatory cytokine) levels. Ciprofloxacin decreased plasma and tissue IL-6 compared to vehicle controls but did not affect MCP-1 or IL-10 levels. To elucidate antibacterial and anti-biofilm metabolite(s) in LaP, fractionation followed by P. aeruginosa antagonistic activity assays were performed. This was followed by liquid chromatography coupled to mass spectrometry (LCMS) analysis. Our analyses identified a low molecular weight, polar molecule which had both antibacterial and anti-biofilm activity.
L. acidophilus secretes an antibacterial and anti-biofilm metabolite that reduced pathogen burden, resolved systemic inflammation in a P. aeruginosa wound infection model.
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Details
- Title
- Lactobacillus acidophilus (strain Scav) Postbiotic Metabolites Reduce Infection and Modulate Inflammation in an in vivo model of Pseudomonas aeruginosa wound infection
- Creators
- Rachael M Wilson - Virtua HealthJean M Walker - Virtua HealthJoris Beld - Drexel UniversityKingsley Yin (Corresponding Author) - Virtua Health
- Publication Details
- Journal of applied microbiology, v 136(3), lxaf061
- Publisher
- Oxford University Press
- Number of pages
- 15
- Grant note
- National Institute of Allergy and Infectious Diseases: 2020-1254
The wound infection study (Protocol #2020-1254) was reviewed and approved by the Institutional Animal Care and Use Committee (IACUC).
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:001454637700001
- Scopus ID
- 2-s2.0-105001699394
- Other Identifier
- 991022040182204721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology