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Late signals from CD27 prevent Fas-dependent apoptosis of primary CD8(+) T cells
Journal article   Open access   Peer reviewed

Late signals from CD27 prevent Fas-dependent apoptosis of primary CD8(+) T cells

Douglas V. Dolfi, Alina C. Boesteanu, Constantinos Petrovas, Dong Xia, Eric A. Butz and Peter D. Katsikis
The Journal of immunology (1950), v 180(5), pp 2912-2921
01 Mar 2008
PMID: 18292513
url
http://www.jimmunol.org/content/180/5/2912.full.pdfView
Published, Version of Record (VoR) Open
url
https://doi.org/10.4049/jimmunol.180.5.2912View
Published, Version of Record (VoR) Open

Abstract

Immunology Life Sciences & Biomedicine Science & Technology
The role of costimulation has previously been confined to the very early stages of the CD8(+) T cell response. In this study, we demonstrate the requirement for CD27 costimulation during the later phase, but not programming of the primary CD8(+) T cell response to influenza virus and reveal a novel mechanism of action for CD27 costimulation. CD27 signals, during the later phase of the primary CD8(+) T cell response, prevent apoptosis of Ag-specific CD8(+) T cells. Blocking CD27L (CD70) on days 6 and 8 after infection reduces the number of NP(366-374)-specific CD8(+) T cells, increases their sensitivity to CD95/Fas-mediated apoptosis, and up-regulates FasL on CD4(+) T cells. This reduction of NP(366-374)-specific CD8(+) T cells requires the presence of CD4(+) T cells and Fas signaling. Lack of CD27 signals also decreases the quality of memory CD8(+) T cell responses. Memory CD8(+) T cells, which express surface CD27 similar to naive cells, however, do not require CD27 costimulation during a secondary response. Thus, CD27 acts indirectly to regulate primary Ag-specific CD8(+) T cell responses by preventing apoptosis of CD8(+) T cells during the later phase of the primary response and is required for optimal quality of memory cells, but is not required during normally primed secondary CD8(+) T cell responses.

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