Journal article
Layered long-term co-culture of hepatocytes and endothelial cells on a transwell membrane: toward engineering the liver sinusoid
Biofabrication, v 5(4), pp 045008-045008
26 Nov 2013
PMID: 24280542
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This paper presents a novel liver model that mimics the liver sinusoid where most liver activities occur. A key aspect of our current liver model is a layered co-culture of primary rat hepatocytes (PRHs) and primary rat liver sinusoidal endothelial cells (LSECs) or bovine aortic endothelial cells (BAECs) on a transwell membrane. When a layered co-culture was attempted with a thin Matrigel layer placed between hepatocytes and endothelial cells to mimic the space of Disse, the cells did not form completely separated monolayers. However, when hepatocytes and endothelial cells were cultured on the opposite sides of a transwell membrane, PRHs co-cultured with LSECs or BAECs maintained their viability and normal morphology for 39 and 57 days, respectively. We assessed the presence of hepatocyte-specific differentiation markers to verify that PRHs remained differentiated in the long-term co-culture and analyzed hepatocyte function by monitoring urea synthesis. We also noted that the expression of cytochrome P-450 remained similar in the co-cultured system from day 1 to day 48. Thus, our novel liver model system demonstrated that primary hepatocytes can be cultured for extended times and retain their hepatocyte-specific functions when layered with endothelial cells.
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Details
- Title
- Layered long-term co-culture of hepatocytes and endothelial cells on a transwell membrane: toward engineering the liver sinusoid
- Creators
- Young Bok (Abraham) Kang - Drexel University Mechanical Engineering and Mechanics, Philadelphia, PA, USASiddhartha Rawat - Drexel University College of Medicine Graduate Program in Molecular and Cellular Biology and Genetics, Philadelphia, PA, USAJoseph Cirillo - Drexel University Mechanical Engineering and Mechanics, Philadelphia, PA, USAMichael Bouchard - Drexel University College of Medicine Biochemistry and Molecular Biology, Philadelphia, PA, USAHongseok (Moses) Noh - Drexel University Mechanical Engineering and Mechanics, Philadelphia, PA, USA
- Publication Details
- Biofabrication, v 5(4), pp 045008-045008
- Publisher
- IOP Publishing
- Number of pages
- 12
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000327886100009
- Scopus ID
- 2-s2.0-84889026967
- Other Identifier
- 991014878311304721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials