Journal article
Leishmania: Naive Human T Cells Sensitized with Promastigote Antigen and IL-12 Develop into Potent Th1 and CD8+ Cytotoxic Effectors
Experimental parasitology, v 93(3), pp 161-170
01 Nov 1999
PMID: 10529358
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Russo, D. M., Chakrabarti, P., and Higgins, A. Y. 1999. Leishmania: Naive human T cells sensitized with promastigote antigen and IL-12 develop into potent Th1 and CD8+ cytotoxic effectors. Experimental Parasitology93, 161–170. The differentiation of naive human T cells into Leishmania-specific Th1 or cytotoxic effector cells was examined by sensitizing T cells in vitro with dead Leishmania antigen in the presence or absence of IFN-γ or IL-12. These Leishmania-specific T cell lines proliferated and produced cytokines in response to challenge with autologous Leishmania-infected macrophages. Sensitization in the presence of IL-12 or IFN-γ induced Leishmania-specific human Th1 responses, with IL-12 inducing more potent Th1 responses. However, IL-12-induced Th1 responses were IFN-γ dependent. T cell lines exhibited Th2 or Th0 phenotypes when primed in the absence of cytokines. Only T cell lines primed in the presence of IL-12 contained high percentages of CD8+ cells. These cells lysed autologous Leishmania-infected but not uninfected macrophages in an MHC-dependent manner. Thus, this in vitro sensitization system can be used to delineate the conditions for optimally priming human Leishmania-specific effector cells.
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Details
- Title
- Leishmania: Naive Human T Cells Sensitized with Promastigote Antigen and IL-12 Develop into Potent Th1 and CD8+ Cytotoxic Effectors
- Creators
- Donna M. Russo - Meharry Medical CollegePampa Chakrabarti - Meharry Medical CollegeAngelica Y. Higgins - Meharry Medical College
- Publication Details
- Experimental parasitology, v 93(3), pp 161-170
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- MD (Doctor of Medicine) Program
- Web of Science ID
- WOS:000083677100005
- Scopus ID
- 2-s2.0-0033230519
- Other Identifier
- 991020830855004721
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- Web of Science research areas
- Parasitology