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Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells
Journal article   Open access   Peer reviewed

Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells

Xiao Huang, Qirui Hu, Yifan Lai, Demosthenes P. Morales, Dennis O. Clegg and Norbert O. Reich
Advanced materials (Weinheim), v 28(48), pp 10732-10737
01 Dec 2016
PMID: 27787919
url
https://escholarship.org/content/qt8k28m2j9/qt8k28m2j9.pdfView
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Abstract

Chemistry Chemistry, Multidisciplinary Chemistry, Physical Materials Science, Multidisciplinary Nanoscience & Nanotechnology Physics, Applied Physics, Condensed Matter Science & Technology Science & Technology - Other Topics Materials Science Physical Sciences Physics Technology
A new method of spatially controlled gene regulation in 3D-cultured human embryonic stem cells is developed using hollow gold nanoshells (HGNs) and near-infrared (NIR) light. Targeted cell(s) are discriminated from neighboring cell(s) by focusing NIR light emitted from a two-photon microscope. Irradiation of cells that have internalized HGNs releases surface attached siRNAs and leads to concomitant gene downregulation.

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#3 Good Health and Well-Being

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Web of Science research areas
Chemistry, Multidisciplinary
Chemistry, Physical
Materials Science, Multidisciplinary
Nanoscience & Nanotechnology
Physics, Applied
Physics, Condensed Matter
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