Files and links (2)
Published, Version of Record (VoR)Open Access (License Unspecified), Open
Journal article
Limited availability of ZBP1 restricts axonal mRNA localization and nerve regeneration capacity
The EMBO journal, v 30(22), pp 4665-4677
16 Nov 2011
PMID: 21964071
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Subcellular localization of mRNAs is regulated by RNA-protein interactions. Here, we show that introduction of a reporter mRNA with the 30UTR of beta-actin mRNA competes with endogenous mRNAs for binding to ZBP1 in adult sensory neurons. ZBP1 is needed for axonal localization of beta-actin mRNA, and introducing GFP with the 30UTR of beta-actin mRNA depletes axons of endogenous beta-actin and GAP-43 mRNAs and attenuates both in vitro and in vivo regrowth of severed axons. Consistent with limited levels of ZBP1 protein in adult neurons, mice heterozygous for the ZBP1 gene are haploinsufficient for axonal transport of beta-actin and GAP-43 mRNAs and for regeneration of peripheral nerve. Exogenous ZBP1 can rescue the RNA transport deficits, but the axonal growth deficit is only rescued if the transported mRNAs are locally translated. These data support a direct role for ZBP1 in transport and translation of mRNA cargos in axonal regeneration in vitro and in vivo.
Metrics
Details
- Title
- Limited availability of ZBP1 restricts axonal mRNA localization and nerve regeneration capacity
- Creators
- Christopher J. Donnelly - University of DelawareDianna E. Willis - Burke Medical Research InstituteMei Xu - Emory UniversityChhavy Tep - The Ohio State UniversityChunsu Jiang - Emory UniversitySoonmoon Yoo - Alfred I. duPont Hospital for ChildrenN. Carolyn Schanen - University of DelawareCatherine B. Kirn-Safran - University of DelawareJan van Minnen - University of CalgaryArthur English - AID AtlantaSung Ok Yoon - The Ohio State UniversityGary J. Bassell - Emory UniversityJeffery L. Twiss - Drexel University
- Publication Details
- The EMBO journal, v 30(22), pp 4665-4677
- Publisher
- Wiley
- Number of pages
- 13
- Grant note
- P20RR015588 / NATIONAL CENTER FOR RESEARCH RESOURCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) Adelson Medical Research Foundation K99NR010797 / NATIONAL INSTITUTE OF NURSING RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Nursing Research (NINR) R21NS045880 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) R21-NS045880; R01-NS049041; R01-NS041596; K99-NR010797; R21-NS060098; R01-NS057190; R01-NS039472; P30-NS045758; P20-RR15588 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Nemours Foundation TB2-0602 / Christopher and Dana Reeve Foundation P20-RR020173 / COBRE
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000297691500013
- Scopus ID
- 2-s2.0-81255134129
- Other Identifier
- 991021892011204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology