Journal article
Linkage-Specific in Situ Sialic Acid Derivatization for N-Glycan Mass Spectrometry Imaging of Formalin-Fixed Paraffin-Embedded Tissues
Analytical chemistry (Washington), v 88(11), pp 5904-5913
07 Jun 2016
PMID: 27145236
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging is a rapidly evolving field in which mass spectrometry techniques are applied directly on tissues to characterize the spatial distribution of various molecules such as lipids, protein/peptides, and recently also N-glycans. Glycans are involved in many biological processes and several glycan changes have been associated with different kinds of cancer, making them an interesting target group to study. An important analytical challenge for the study of glycans by MALDI mass spectrometry is the labile character of sialic acid groups which are prone to in-source/postsource decay, thereby biasing the recorded glycan profile. We therefore developed a linkage-specific sialic acid derivatization by dimethylamidation and subsequent amidation and transferred this onto formalin-fixed paraffin-embedded (FFPE) tissues for MALDI imaging of N-glycans. Our results show (i) the successful stabilization of sialic acids in a linkage specific manner, thereby not only increasing the detection range, but also adding biological meaning, (ii) that no noticeable lateral diffusion is induced during to sample preparation, (iii) the potential of mass spectrometry imaging to spatially characterize the N-glycan expression within heterogeneous tissues.
Metrics
Details
- Title
- Linkage-Specific in Situ Sialic Acid Derivatization for N-Glycan Mass Spectrometry Imaging of Formalin-Fixed Paraffin-Embedded Tissues
- Creators
- Stephanie Holst - Leiden University Medical CenterBram Heijs - Leiden University Medical CenterNoortje de Haan - Leiden University Medical CenterRene J. M. van Zeijl - Leiden University Medical CenterInge H. Briaire-de Bruijn - Leiden UniversityGabi W. van Pelt - Leiden University Medical CenterAnand S. Mehta - Drexel UniversityPeggy M. Angel - Medical University of South CarolinaWilma E. Mesker - Leiden University Medical CenterRob A. Tollenaar - Leiden UniversityRichard R. Drake - Medical University of South CarolinaJudith V. M. G. Bovee - Leiden University Medical CenterLiam A. McDonnell - Leiden University Medical CenterManfred Wuhrer - Leiden University Medical Center
- Publication Details
- Analytical chemistry (Washington), v 88(11), pp 5904-5913
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 10
- Grant note
- 305479 / IBD-BIOM R01 CA120206; U01 CA168856 / National Cancer Institute (NCI); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) 93512002 / ZonMW Zenith Project Imaging Mass Spectrometry-Based Molecular Histology: Differentiation and Characterization of Clinically Challenging Soft Tissue Sarcomas Grant 278535 / European Union Seventh Framework Programmes HighGlycan Project Hepatitis B Foundation state of South Carolina SmartState Endowed Research Program U01CA168856 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) R21CA186799 / National Institutes of Health/National Cancer Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000377631000051
- Scopus ID
- 2-s2.0-84974588124
- Other Identifier
- 991019318926204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Analytical