Journal article
Lipid flip-flop vs. lateral diffusion in the relaxation of hemifusion diaphragms
Biochimica et biophysica acta. Biomembranes, v 1860(7), pp 1452-1459
Jul 2018
PMID: 29684332
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Molecular dynamics simulations of a solvent-free coarse-grained lipid model are used to characterize the mechanisms by which lipid-bilayer hemifusion diaphragm (HD) intermediates relax, across a range of global compositions of negative intrinsic curvature (NIC) lipids and neutral-curvature lipids. At low concentrations of NIC lipids, rapid fission produces a double bilayer end state through a lateral diffusion-based mechanism enabled by spontaneous rim-pore defects. At moderately higher NIC lipid concentrations, rim pores are absent and stable leaflet three-junctions persist, revealing an HD relaxation mechanism entirely reliant on lipid flip-flop, and end states that are either stable fusion pores or stable HD's. These fusogenic systems exhibit dynamics highly dependent on NIC lipid concentration via an underlying sensitivity of flip-flop rates for neutral lipids on NIC lipid concentration. This work illustrates that HD dynamics may be altered through regulation of lipid composition in the immediate three-junction region. This work further highlights the potential role of flippases in biological fusion and the importance of lipid composition on fusion dynamics.
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Details
- Title
- Lipid flip-flop vs. lateral diffusion in the relaxation of hemifusion diaphragms
- Creators
- Jasmine M Gardner - Department of Chemical and Biological Engineering, Drexel University, 3141 Chestnut St., Philadelphia, PA 19104, United StatesCameron F Abrams - Department of Chemical and Biological Engineering, Drexel University, 3141 Chestnut St., Philadelphia, PA 19104, United States. Electronic address: cfa22@drexel.edu
- Publication Details
- Biochimica et biophysica acta. Biomembranes, v 1860(7), pp 1452-1459
- Publisher
- Elsevier; Netherlands
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000433642100004
- Scopus ID
- 2-s2.0-85046170957
- Other Identifier
- 991014877707904721
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InCites Highlights
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- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics