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Journal article
Lipid nanoparticles (LNP) induce activation and maturation of antigen presenting cells in young and aged individuals
Communications biology, v 6(1), pp 188-188
17 Feb 2023
PMID: 36805684
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Herein, we studied the impact of empty LNP (eLNP), component of mRNA-based vaccine, on anti-viral pathways and immune function of cells from young and aged individuals. eLNP induced maturation of monocyte derived dendritic cells (MDDCs). We further show that eLNP upregulated CD40 and induced cytokine production in multiple DC subsets and monocytes. This coincided with phosphorylation of TANK binding kinase 1 (pTBK1) and interferon response factor 7 (pIRF7). In response to eLNP, healthy older adults (>65 yrs) have decreased CD40 expression, and IFN-γ output compared to young adults (<65 yrs). Additionally, cells from older adults have a dysregulated anti-viral signaling response to eLNP stimulation, measured by the defect in type I IFN production, and phagocytosis. Overall, our data show function of eLNP in eliciting DC maturation and innate immune signaling pathways that is impaired in older adults resulting in lower immune responses to SARS-CoV-2 mRNA-based vaccines.
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Details
- Title
- Lipid nanoparticles (LNP) induce activation and maturation of antigen presenting cells in young and aged individuals
- Creators
- Jennifer Connors - Drexel UniversityDavid Joyner - Drexel UniversityNathan J Mege - Reading HospitalGina M Cusimano - Drexel UniversityMatthew R Bell - Drexel UniversityJennifer Marcy - Drexel UniversityBhavani Taramangalam - Drexel UniversityKenneth M Kim - Drexel UniversityPaulo J C Lin - Acuitas Therapeutics (Canada)Ying K Tam - Acuitas Therapeutics (Canada)Drew Weissman - University of PennsylvaniaMichele A Kutzler - Drexel UniversityMohamad-Gabriel Alameh - University of PennsylvaniaElias K Haddad - Drexel University
- Publication Details
- Communications biology, v 6(1), pp 188-188
- Publisher
- Springer Nature
- Number of pages
- 1
- Grant note
- 1U19 AI128910-01 / Division of Intramural Research, National Institute of Allergy and Infectious Diseases (Division of Intramural Research of the NIAID)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Medicine (Graduate); Infectious Diseases (and HIV Medicine)
- Web of Science ID
- WOS:000935894600003
- Scopus ID
- 2-s2.0-85148347180
- Other Identifier
- 991020150931704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biology