Journal article
Liver Is a Generative Site for the B Cell Response to Ehrlichia muris
Immunity (Cambridge, Mass.), v 51(6), pp 1088-1101.e5
17 Dec 2019
PMID: 31732168
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The B cell response to Ehrlichia muris is dominated by plasmablasts (PBs), with few-if any-germinal centers (GCs), yet it generates protective immunoglobulin M (IgM) memory B cells (MBCs) that express the transcription factor T-bet and harbor V-region mutations. Because Ehrlichia prominently infects the liver, we investigated the nature of liver B cell response and that of the spleen. B cells within infected livers proliferated and underwent somatic hypermutation (SHM). Vh-region sequencing revealed trafficking of clones between the spleen and liver and often subsequent local clonal expansion and intraparenchymal localization of T-bet(+) MBCs. T-bet(+) MBCs expressed MBC subset markers CD80 and PD-L2. Many T-ber MBCs lacked CD11b or CD11c expression but had marginal zone (MZ) B cell phenotypes and colonized the splenic MZ, revealing T-bet(+) MBC plasticity. Hence, liver and spleen are generative sites of B cell responses, and they include V-region mutation and result in liver MBC localization.
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Details
- Title
- Liver Is a Generative Site for the B Cell Response to Ehrlichia muris
- Creators
- Nikita Trivedi - University of PittsburghFlorian Weisel - University of PittsburghShuchi Smita - University of PittsburghStephen Joachim - University of PittsburghMuhamuda Kader - University of PittsburghAditya Radhakrishnan - Juno Therapeutics, Seattle, WA 98109, USAChris Clouser - Juno Therapeut, Seattle, WA 98109 USAAaron M. Rosenfeld - Drexel UniversityMaria Chikina - University of PittsburghFrancois Vigneault - Juno Therapeutics, Seattle, WA 98109, USAUri Hershberg - Drexel UniversityNahed Ismail - University of PittsburghMark Jay Shlomchik - University of Pittsburgh
- Publication Details
- Immunity (Cambridge, Mass.), v 51(6), pp 1088-1101.e5
- Publisher
- Elsevier
- Number of pages
- 19
- Grant note
- NIH-1R01AI105018; NIH-2R01AI043603; P01-AI106697 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01AI105018 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000503162900014
- Scopus ID
- 2-s2.0-85076144941
- Other Identifier
- 991019167544204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology