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Journal article
Liver sinusoid on a chip: Long-term layered co-culture of primary rat hepatocytes and endothelial cells in microfluidic platforms
Biotechnology and bioengineering, v 112(12), pp 2571-2582
Dec 2015
PMID: 25994312
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We describe the generation of microfluidic platforms for the co-culture of primary hepatocytes and endothelial cells; these platforms mimic the architecture of a liver sinusoid. This paper describes a progressional study of creating such a liver sinusoid on a chip system. Primary rat hepatocytes (PRHs) were co-cultured with primary or established endothelial cells in layers in single and dual microchannel configurations with or without continuous perfusion. Cell viability and maintenance of hepatocyte functions were monitored and compared for diverse experimental conditions. When primary rat hepatocytes were co-cultured with immortalized bovine aortic endothelial cells (BAECs) in a dual microchannel with continuous perfusion, hepatocytes maintained their normal morphology and continued to produce urea for at least 30 days. In order to demonstrate the utility of our microfluidic liver sinusoid platform, we also performed an analysis of viral replication for the hepatotropic hepatitis B virus (HBV). HBV replication, as measured by the presence of cell-secreted HBV DNA, was successfully detected. We believe that our liver model closely mimics the in vivo liver sinusoid and supports long-term primary liver cell culture. This liver model could be extended to diverse liver biology studies and liver-related disease research such as drug induced liver toxicology, cancer research, and analysis of pathological effects and replication strategies of various hepatotropic infectious agents. .
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Details
- Title
- Liver sinusoid on a chip: Long-term layered co-culture of primary rat hepatocytes and endothelial cells in microfluidic platforms
- Creators
- Young Bok Abraham Kang - Drexel UniversityTemitope R Sodunke - Drexel UniversityJason Lamontagne - Drexel UniversityJoseph Cirillo - Drexel UniversityCaroline Rajiv - Drexel UniversityMichael J Bouchard - Drexel UniversityMoses Noh - Drexel University
- Publication Details
- Biotechnology and bioengineering, v 112(12), pp 2571-2582
- Publisher
- Wiley
- Grant note
- 1R21AA017458-01A2 / NIAAA NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000363780500016
- Scopus ID
- 2-s2.0-84946476258
- Other Identifier
- 991019167968304721
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- Web of Science research areas
- Biotechnology & Applied Microbiology