Journal article
Localization of amiloride-sensitive sodium channels in A6 cells by atomic force microscopy
American Journal of Physiology: Cell Physiology, v 272(4), pp C1295-C1298
01 Apr 1997
PMID: 9142855
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Atomic force microscopy (AFM) was used for high-resolution imaging of the apical distribution of epithelial Na+ channels in A6 renal epithelial cells. A6 cells grown on coverslips were labeled with antibodies generated against an amiloride-sensitive epithelial Na+ channel complex purified from bovine renal medulla that had been conjugated to 8-nm colloidal gold particles before preparation for AFM. AFM revealed that there was a marked increase in the height of the microvilli in cells labeled with the anti-epithelial Na+ channel antibodies compared with unlabeled cells or cells labeled with rabbit nonimmune immunoglobulin G conjugated to colloidal gold particles. We interpret this apparent increase in microvillar height to be due to anti-epithelial Na+ channel antibody binding to the apical microvilli. These data demonstrate that epithelial Na+ channels are restricted to the apical microvilli in Na+-transporting renal epithelial cells. Furthermore, they demonstrate the applicability of using AFM for high-resolution imaging of the cell surface distribution of epithelial ion channels.
Metrics
Details
- Title
- Localization of amiloride-sensitive sodium channels in A6 cells by atomic force microscopy
- Creators
- P. R. Smith - Drexel UniversityA. L. Bradford - University of Alabama at BirminghamS. Schneider - Yale UniversityD. J. Benos - University of Alabama at BirminghamJ. P. Geibel - Yale University
- Publication Details
- American Journal of Physiology: Cell Physiology, v 272(4), pp C1295-C1298
- Publisher
- American Physiological Society (APS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine; Medical College of Pennsylvania and Hahnemann University (1993-1996, 1998-2002)
- Web of Science ID
- WOS:A1997WU49700025
- Scopus ID
- 2-s2.0-0030962643
- Other Identifier
- 991019168090404721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology
- Physiology