Journal article
Localization of arginine-rich protein in cytomegalovirus dense bodies using phenanthrenequinone fluorescence
Virology (New York, N.Y.), v 96(1), pp 335-338
15 Jul 1979
PMID: 88802
Abstract
Phenanthrenequinone (PQ) is a useful reagent for the intracellular localization of arginine-rich proteins and polypeptides by fluorescence microscopy. In WI-38 cells infected with human cytomegalovirus (CMV), PQ staining revealed an intense fluorescence of the so-called cytoplasmic dense bodies, indicating that one or more dense body proteins are rich in arginine. Since previous biochemical studies have shown that many dense body proteins appear identical to viral structural proteins, the present findings also suggest that human CMV, like HSV, may contain one or more arginine-rich structural proteins. In HSV-infected cells, where it is known that dense bodies are not formed, no fluorescent cytoplasmic bodies were observed after PQ treatment. The ability of PQ staining to distinguish between HSV- and CMV-infected cells suggests that this technique may be a potentially useful adjunct method in the diagnosis of certain clinical CMV infections.
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Details
- Title
- Localization of arginine-rich protein in cytomegalovirus dense bodies using phenanthrenequinone fluorescence
- Creators
- Janet Duyckinck Smith - Drexel UniversityEtienne de Harven - Drexel University
- Publication Details
- Virology (New York, N.Y.), v 96(1), pp 335-338
- Publisher
- Elsevier; SAN DIEGO
- Number of pages
- 4
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1979HD92300042
- Scopus ID
- 2-s2.0-0018776874
- Other Identifier
- 991021900202004721