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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Location of Biomarkers and Reagents within Agarose Beads of a Programmable Bio-nano-chip
Small (Weinheim an der Bergstrasse, Germany), v 7(5), pp 613-624
07 Mar 2011
PMCID: PMC3397282
PMID: 21290601
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The slow development of cost-effective medical microdevices with strong analytical performance characteristics is due to a lack of selective and efficient analyte capture and signaling. The recently developed programmable bio-nano-chip (PBNC) is a flexible detection device with analytical behavior rivaling established macroscopic methods. The PBNC system employs approximate to 300 mu m-diameter bead sensors composed of agarose "nanonets" that populate a microelectromechanical support structure with integrated microfluidic elements. The beads are an efficient and selective protein-capture medium suitable for the analysis of complex fluid samples. Microscopy and computational studies probe the 3D interior of the beads. The relative contributions that the capture and detection of moieties, analyte size, and bead porosity make to signal distribution and intensity are reported. Agarose pore sizes ranging from 45 to 620 nm are examined and those near 140 nm provide optimal transport characteristics for rapid (< 15 min) tests. The system exhibits efficient (99.5%) detection of bead-bound analyte along with low (approximate to 2%) nonspecific immobilization of the detection probe for carcinoembryonic antigen assay. Furthermore, the role analyte dimensions play in signal distribution is explored, and enhanced methods for assay building that consider the unique features of biomarker size are offered.
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Details
- Title
- Location of Biomarkers and Reagents within Agarose Beads of a Programmable Bio-nano-chip
- Creators
- Jesse V. Jokerst - The University of Texas at AustinJie Chou - Rice UniversityJames P. Camp - The University of Texas at AustinJorge Wong - The University of Texas at AustinAlexis Lennart - The University of Texas at AustinAmanda A. Pollard - The University of Texas at AustinPierre N. Floriano - Rice UniversityNicolaos Christodoulides - Rice UniversityGlennon W. Simmons - Rice UniversityYanjie Zhou - The University of Texas at AustinMehnaaz F. Ali - The University of Texas at AustinJohn T. McDevitt - Rice UniversityJuliana Wong - Medicine (Graduate)
- Publication Details
- Small (Weinheim an der Bergstrasse, Germany), v 7(5), pp 613-624
- Publisher
- Wiley
- Number of pages
- 12
- Grant note
- U01 DE15017; U01 DE017793 / National Institutes of Health through the National Institute of Dental and Craniofacial Research; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) U01DE017793 / NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) U01DE015017 / NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Medicine (Graduate)
- Web of Science ID
- WOS:000288081900012
- Scopus ID
- 2-s2.0-79952232500
- Other Identifier
- 991019173439004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology
- Physics, Applied
- Physics, Condensed Matter