Journal article
Locus Control Region Transcription Plays an Active Role in Long-Range Gene Activation
Molecular cell, v 23(3), pp 365-375
2006
PMID: 16885026
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Activation of eukaryotic genes often relies on remote chromatin determinants. How these determinants function remains poorly understood. The
hGH gene is activated by a 5′-remote locus control region (LCR). Pituitary-specific DNase I hypersensitive site I (HSI), the dominant
hGH LCR element, is separated from the
hGH-N promoter by a 14.5 kb span that encompasses the B-lymphocyte-specific
CD79b gene. Here, we describe a domain of noncoding Pol II transcription in pituitary somatotropes that includes the
hGH LCR and adjacent
CD79b locus. This entire “LCR domain of transcription” is HIS dependent and terminates 3′ to
CD79b, leaving a gap in transcription between this domain and the target
hGH-N promoter. Insertion of a Pol II terminator within the LCR blocks
CD79b transcription and represses
hGH-N expression. These data document an essential role for LCR transcription in long-range control, link “bystander”
CD79b transcription to this process, and support a unique model for locus activation.
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Details
- Title
- Locus Control Region Transcription Plays an Active Role in Long-Range Gene Activation
- Creators
- Yugong Ho - Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104Felice Elefant - Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104Stephen A Liebhaber - Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104Nancy E Cooke - Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
- Publication Details
- Molecular cell, v 23(3), pp 365-375
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000239805700008
- Scopus ID
- 2-s2.0-33746423865
- Other Identifier
- 991014877837404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology