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Journal article
Long-Term IGF-I Exposure Decreases Autophagy and Cell Viability
PloS one, v 5(9), pp 1-10
07 Sep 2010
PMID: 20830296
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A reduction in IGF-I signaling has been found to increase lifespan in multiple organisms despite the fact that IGF-I is a trophic factor for many cell types and has been found to have protective effects against multiple forms of damage in acute settings. The increase in longevity seen in response to reduced IGF-I signaling suggests that there may be differences between the acute and chronic impact of IGF-I signaling. We have examined the possibility that long-term stimulation with IGF-I may have a negative impact at the cellular level using quiescent human fibroblasts. We find that fibroblast cells exposed to IGF-I for 14 days have reduced long-term viability as judged by colony forming assays, which is accompanied by an accumulation of senescent cells. In addition we observe an accumulation of cells with depolarized mitochondria and a reduction in autophagy in the long-term IGF-I treated cultures. An examination of mice with reduced IGF-I levels reveals evidence of enhanced autophagy and fibroblast cells derived from these mice have a larger mitochondrial mass relative to controls indicating that changes in mitochondrial turnover occurs in animals with reduced IGF-I. The results indicate that chronic IGF-I stimulation leads to mitochondrial dysfunction and reduced cell viability.
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Details
- Title
- Long-Term IGF-I Exposure Decreases Autophagy and Cell Viability
- Creators
- Alessandro Bitto - Drexel UniversityChad Lerner - Drexel UniversityClaudio Torres - Drexel UniversityMichaela Roell - University of BolognaMarco Malaguti - University of BolognaViviana Perez - The University of Texas Health Science Center at San AntonioAntonello Lorenzini - University of BolognaSilvana Hrelia - The University of Texas Health Science Center at San AntonioYuji Ikeno - Audie L. Murphy Memorial VA HospitalMichelle Elizabeth Matzko - Pennsylvania State UniversityRoger McCarter - The University of Texas Health Science Center at San AntonioChristian Sell - Drexel University
- Publication Details
- PloS one, v 5(9), pp 1-10
- Publisher
- Public Library of Science
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Neurobiology and Anatomy
- Web of Science ID
- WOS:000281631300020
- Scopus ID
- 2-s2.0-77958611244
- Other Identifier
- 991019168872704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology