Journal article
Long-term Sudan Virus Ebola Survivors Maintain Multiple Antiviral Defense Mechanisms
The Journal of infectious diseases, v 230(2), pp 426-437
16 Aug 2024
PMID: 38066574
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background The critical issues of sustained memory immunity following ebolavirus disease among long-term survivors are still unclear.Methods Here, we examine virus-specific immune and inflammatory responses following in vitro challengd in 12 Sudan virus (SUDV) long-term survivors from Uganda's 2000-2001 Gulu outbreak, 15 years after recovery. Total RNA from isolated SUDV-stimulated and unstimulated peripheral blood mononuclear cells was extracted and analyzed. Matched serum samples were also collected to determine SUDV IgG levels and functionality.Results We detected persistent humoral (58%, 7 of 12) and cellular (33%, 4 of 12) immune responses in SUDV long-term survivors and identified critical molecular mechanisms of innate and adaptive immunity. Gene expression in immune pathways, the interferon signaling system, antiviral defense response, and activation and regulation of T- and B-cell responses were observed. SUDV long-term survivors also maintained robust virus-specific IgG antibodies capable of polyfunctional responses, including neutralizing and innate Fc effector functions.Conclusions Data integration identified significant correlations among humoral and cellular immune responses and pinpointed a specific innate and adaptive gene expression signature associated with long-lasting immunity. This could help identify natural and vaccine correlates of protection against ebolavirus disease.
Our study in naturally recovered long-term Sudan virus survivors revealed durable polyfunctional humoral and cellular memory immune responses with distinctive gene expression signatures, which may provide long-lasting protective immunity and help to define the ebolavirus correlate of protection.
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Details
- Title
- Long-term Sudan Virus Ebola Survivors Maintain Multiple Antiviral Defense Mechanisms
- Creators
- Ariel Sobarzo - Ben-Gurion University of the NegevYves Mone - Drexel University, Microbiology and ImmunologySteven Lang - Drexel Univ, Coll Med, Ctr Genom Sci, Dept Microbiol & Immunol, Philadelphia, PA USASigal Gelkop - Ben-Gurion University of the NegevPolina Brangel - London Centre for NanotechnologyAna Kuehne - United States Army Medical Research Institute of Infectious DiseasesRachel A. Mckendry - London Centre for NanotechnologyJoshua Chang Mell - Drexel University, Microbiology and ImmunologyAzad Ahmed - Drexel University, Microbiology and ImmunologyClaytus Davis - Ben-Gurion University of the NegevJohn M. Dye - United States Army Medical Research Institute of Infectious DiseasesJulius Julian Lutwama - Uganda Virus Research InstituteLeslie Lobel - Ben-Gurion University of the NegevFrancisco Veas - Université de MontpellierGarth D. Ehrlich (Corresponding Author) - Drexel University, Microbiology and Immunology
- Publication Details
- The Journal of infectious diseases, v 230(2), pp 426-437
- Publisher
- Oxford University Press
- Number of pages
- 12
- Grant note
- 666102 / European Commission; European Union (EU); European Commission Joint Research Centre MR/P024378/1 / Medical Research Council; UK Research & Innovation (UKRI); Medical Research Council UK (MRC) Center for Advanced Microbial Processing Center for Genomic Sciences within the Institute for Molecular Medicine and Infectious Disease at Drexel University MR/P024378/1 / MRC; UK Research & Innovation (UKRI); Medical Research Council UK (MRC) EP/K031953/1 / Engineering and Physical Sciences Research Council; UK Research & Innovation (UKRI); Engineering & Physical Sciences Research Council (EPSRC) EP/K031953/1; EP/R00529X/1 / EPSRC; UK Research & Innovation (UKRI); Engineering & Physical Sciences Research Council (EPSRC) 666102 / European Research Council (ERC) RIA-2016E-1609 / PANDORA-ID-Net project 101046084 / EPIC-CROWN2 Horizon Europe
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:001135425800001
- Scopus ID
- 2-s2.0-85201438886
- Other Identifier
- 991021811749404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Immunology
- Infectious Diseases
- Microbiology