Journal article
Longitudinal On-Column Thermal Modulation for Comprehensive Two-Dimensional Liquid Chromatography
Analytical chemistry (Washington), v 89(2), pp 1123-1130
17 Jan 2017
PMID: 28194983
Abstract
Longitudinal on-column thermal modulation for comprehensive two-dimensional liquid chromatography is introduced. Modulation optimization involved a systematic investigation of heat transfer, analyte retention, and migration velocity at a range of temperatures. Longitudinal on-column thermal modulation was realized using a set of alkylphenones and compared to a conventional valve-modulator employing sample loops. The thermal modulator showed a reduced modulation-induced pressure impact than valve modulation, resulting in reduced baseline perturbation by a factor of 6; yielding a 6-14-fold improvement in signal-to-noise. A red wine sample was analyzed to demonstrate the potential of the longitudinal on-column thermal modulator for separation of a complex sample. Discrete peaks in the second dimension using the thermal modulator were 30-55% narrower than with the valve modulator. The results shown herein demonstrate the benefits of an active focusing modulator, such as reduced detection limits and increased total peak capacity.
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Details
- Title
- Longitudinal On-Column Thermal Modulation for Comprehensive Two-Dimensional Liquid Chromatography
- Creators
- Mari E Creese - Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania , Private Bag 75, Hobart, Tasmania 7001, AustraliaMathew J Creese - Allison Laboratories Pty Ltd. , Sandy Bay, Tasmania 7005, AustraliaJoe P Foley - Department of Chemistry, Drexel University , 3141 Chestnut Street, Philadelphia 19104, United StatesHernan J Cortes - HJ Cortes Consulting LLC , Midland, Michigan 48642, United StatesEmily F Hilder - Future Industries Institute, University of South Australia , GPO Box 2471, Adelaide, South Australia 5001, AustraliaRobert A Shellie - Trajan Scientific and Medical , 7 Argent Place, Ringwood, Victoria 3134, AustraliaMichael C Breadmore - Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania , Private Bag 75, Hobart, Tasmania 7001, Australia
- Publication Details
- Analytical chemistry (Washington), v 89(2), pp 1123-1130
- Publisher
- American Chemical Society; Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000392458100016
- Scopus ID
- 2-s2.0-85033198190
- Other Identifier
- 991014878106504721
InCites Highlights
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Analytical