Files and links (1)
Accepted (AM)Open Access (License Unspecified), Open
Journal article
Longitudinal analysis of ANA in the Systemic Lupus International Collaborating Clinics (SLICC) Inception Cohort
Annals of the rheumatic diseases, v 81(8), pp 1143-1150
01 Aug 2022
PMID: 35338033
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
ObjectivesA perception derived from cross-sectional studies of small systemic lupus erythematosus (SLE) cohorts is that there is a marked discrepancy between antinuclear antibody (ANA) assays, which impacts on clinicians’ approach to diagnosis and follow-up. We compared three ANA assays in a longitudinal analysis of a large international incident SLE cohort retested regularly and followed for 5 years.MethodsDemographic, clinical and serological data was from 805 SLE patients at enrolment, year 3 and 5. Two HEp-2 indirect immunofluorescence assays (IFA1, IFA2), an ANA ELISA, and SLE-related autoantibodies were performed in one laboratory. Frequencies of positivity, titres or absorbance units (AU), and IFA patterns were compared using McNemar, Wilcoxon and kappa statistics, respectively.ResultsAt enrolment, ANA positivity (≥1:80) was 96.1% by IFA1 (median titre 1:1280 (IQR 1:640–1:5120)), 98.3% by IFA2 (1:2560 (IQR 1:640–1:5120)) and 96.6% by ELISA (176.3 AU (IQR 106.4 AU–203.5 AU)). At least one ANA assay was positive for 99.6% of patients at enrolment. At year 5, ANA positivity by IFAs (IFA1 95.2%; IFA2 98.9%) remained high, while there was a decrease in ELISA positivity (91.3%, p<0.001). Overall, there was >91% agreement in ANA positivity at all time points and ≥71% agreement in IFA patterns between IFA1 and IFA2.ConclusionIn recent-onset SLE, three ANA assays demonstrated commutability with a high proportion of positivity and titres or AU. However, over 5 years follow-up, there was modest variation in ANA assay performance. In clinical situations where the SLE diagnosis is being considered, a negative test by either the ELISA or HEp-2 IFA may require reflex testing.
Metrics
Details
- Title
- Longitudinal analysis of ANA in the Systemic Lupus International Collaborating Clinics (SLICC) Inception Cohort
- Creators
- May Yee Choi - University of CalgaryAnn Elaine Clarke - University of CalgaryMurray Urowitz - Toronto Western HospitalJohn Hanly - Queen Elizabeth II Health Sciences CentreYvan St-Pierre - McGill University Health CentreCaroline Gordon - University of BirminghamSang-Cheol Bae - Hanyang University Seoul HospitalJuanita Romero-Diaz - Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránJorge Sanchez-Guerrero - Toronto Western HospitalSasha Bernatsky - McGill University Health CentreDaniel J Wallace - Cedars-Sinai Medical CenterDavid Isenberg - University College LondonAnisur Rahman - University College LondonJoan T Merrill - Oklahoma Medical Research FoundationPaul R Fortin - Université LavalDafna D Gladman - Toronto Western HospitalIan N Bruce - Manchester Academic Health Science CentreMichelle Petri - Johns Hopkins MedicineEllen M Ginzler - SUNY Downstate Health Sciences UniversityMary Anne Dooley - University of North Carolina at Chapel HillRosalind Ramsey-Goldman - Northwestern UniversitySusan Manzi - University of PittsburghAndreas Jönsen - Lund UniversityGraciela S Alarcón - University of Alabama at BirminghamRonald F van Vollenhoven - University of AmsterdamCynthia Aranow - Feinstein Institute for Medical ResearchMeggan Mackay - Feinstein Institute for Medical ResearchGuillermo Ruiz-Irastorza - BioCruces Health research InstituteSam Lim - Emory UniversityMurat Inanc - Istanbul UniversityKen Kalunian - David Geffen School of Medicine at UCLASøren Jacobsen - Copenhagen University HospitalChristine Peschken - University of ManitobaDiane L Kamen - Medical University of South CarolinaAnca Askanase - New York University Langone Orthopedic HospitalJill P Buyon - New York UniversityKaren H Costenbader - Brigham and Women's HospitalMarvin J Fritzler - University of Calgary
- Publication Details
- Annals of the rheumatic diseases, v 81(8), pp 1143-1150
- Publisher
- BMJ Publishing Group Ltd and European League Against Rheumatism
- Number of pages
- 8
- Grant note
- London Hospitals Biomedical Research Centre Distinguished Senior Investigator of The Arthritis; Research Chair in Rheumatic Diseases at the Univer / Arthritis Society (http://dx.doi.org/10.13039/501100000142) LUPUS UK (http://dx.doi.org/10.13039/501100018817) 1U54TR001353; AR043727; AR046588; AR069572; K24 AR002213; K24 AR066109; K24-AR-02318; P60AR064464; RR00046; UL-1RR-025741 / National Institute for Health Research (http://dx.doi.org/10.13039/501100000272) Gary S Gilkeson Career Development Award / Lupus Foundation of America (http://dx.doi.org/10.13039/100002193) A05990 / Novo Nordisk Foundation A1028 / Gigtforeningen (http://dx.doi.org/10.13039/100008368) Singer Family Fund for Lupus Research National Institute for Health Research Manchester Biomedical Research Centre MOP-88526 / Canadian Institutes of Health Research (http://dx.doi.org/10.13039/501100000024)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- General Internal Medicine
- Web of Science ID
- WOS:000774804600001
- Scopus ID
- 2-s2.0-85134226098
- Other Identifier
- 991021933902204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Rheumatology