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Loss of meiotic rereplication block in Saccharomyces cerevisiae cells defective in Cdc28p regulation
Journal article   Open access

Loss of meiotic rereplication block in Saccharomyces cerevisiae cells defective in Cdc28p regulation

Lyndi M Rice, Constantine Plakas, Joseph T Nickels, Jr and Joseph T Nickels
Eukaryotic cell, v 4(1)
Jan 2005
DOI: https://doi.org/10.1128/EC.4.1.55-62.2005
PMID: 15643060
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1128/ec.4.1.55-62.2005View
Published, Version of Record (VoR)Open Access (License Unspecified),  Open
url
https://doi.org/10.1128/EC.4.1.55-62.2005View
Published, Version of Record (VoR) Open

Abstract

Alleles Blotting, Northern CDC28 Protein Kinase, S cerevisiae - genetics CDC28 Protein Kinase, S cerevisiae - physiology Cell Cycle Proteins - metabolism Cell Separation Cyclin B - metabolism Cyclin-Dependent Kinase Inhibitor Proteins DNA - metabolism DNA Replication DNA-Binding Proteins - metabolism Flow Cytometry Gene Expression Regulation, Fungal Genes, Dominant Green Fluorescent Proteins - metabolism Meiosis Models, Genetic Nuclear Proteins Phenotype Phosphoprotein Phosphatases - metabolism Plasmids - metabolism Protein Phosphatase 2 Protein-Tyrosine Kinases - metabolism Recombination, Genetic RNA - metabolism Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins - metabolism Time Factors
Cdc28p is the major cyclin-dependent kinase in Saccharomyces cerevisiae. Its activity is required for blocking the reinitiation of DNA replication during mitosis. Here, we show that under conditions where Cdc28p activity is improperly regulated--either through the loss of function of the Schizosaccharomyces pombe wee1 ortholog Swe1p or through the expression of a dominant CDC28 allele, CDC28AF--diploid yeast cells are able to complete several rounds of premeiotic DNA replication within a single meiotic cell cycle. Moreover, a percentage of mutant cells exhibit a "multispore" phenotype, possessing the ability to package more than four spores within a single ascus. These multispored asci contain both even and odd numbers of viable spores. In order for meiotic rereplication and multispore formation to occur, cells must initiate homologous recombination and maintain proper chromosome cohesion during meiosis I. Rad9p- or Rad17p-dependent checkpoint mechanisms are not required for multispore formation and neither are the B-type cyclin Clb6p and the cyclin-dependent kinase inhibitor Sic1p. Finally, we present evidence of a possible role for a Cdc55p-dependent protein phosphatase 2A in initiating meiotic replication.

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Web of Science research areas
Microbiology
Mycology
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