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Journal article
Lymphocyte egress signal sphingosine-1-phosphate promotes ERM-guided, bleb-based migration
The Journal of cell biology, v 220(6)
07 Jun 2021
PMID: 33764397
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Ezrin, radixin, and moesin (ERM) family proteins regulate cytoskeletal responses by tethering the plasma membrane to the underlying actin cortex. Mutations in ERM proteins lead to severe combined immunodeficiency, but the function of these proteins in T cells remains poorly defined. Using mice in which T cells lack all ERM proteins, we demonstrate a selective role for these proteins in facilitating S1P-dependent egress from lymphoid organs. ERM-deficient T cells display defective S1P-induced migration in vitro, despite normal responses to standard protein chemokines. Analysis of these defects revealed that S1P promotes a fundamentally different mode of migration than chemokines, characterized by intracellular pressurization and bleb-based motility. ERM proteins facilitate this process, controlling directional migration by limiting blebbing to the leading edge. We propose that the distinct modes of motility induced by S1P and chemokines are specialized to allow T cell migration across lymphatic barriers and through tissue stroma, respectively.
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Details
- Title
- Lymphocyte egress signal sphingosine-1-phosphate promotes ERM-guided, bleb-based migration
- Creators
- Tanner F. Robertson - Children's Hospital of PhiladelphiaPragati Chengappa - Department of Biology Drexel University Philadelphia PADaniela Gomez Atria - Division of Hematology-Oncology, Department of Medicine, Abramson Family Cancer Research Institute, University of Pennsylvania Perelman School of Medicine, Philadelphia, PAChristine F. Wu - Children's Hospital of PhiladelphiaLyndsay Avery - Children's Hospital of PhiladelphiaNathan H. Roy - Children's Hospital of PhiladelphiaIvan Maillard - Division of Hematology-Oncology, Department of Medicine, Abramson Family Cancer Research Institute, University of Pennsylvania Perelman School of Medicine, Philadelphia, PARyan J. Petrie - Drexel UniversityJanis K. Burkhardt - Children's Hospital of Philadelphia
- Publication Details
- The Journal of cell biology, v 220(6)
- Publisher
- Rockefeller Univ Press
- Number of pages
- 22
- Grant note
- R01 HL128551; R01 AI 091627; R01 GM126054; T32 HD083185 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000648761600001
- Scopus ID
- 2-s2.0-85103493120
- Other Identifier
- 991019167788504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology