Journal article
MALDI imaging mass spectrometry profiling of N-glycans in formalin-fixed paraffin embedded clinical tissue blocks and tissue microarrays
PloS one, v 9(9), pp e106255-e106255
2014
PMCID: PMC4153616
PMID: 25184632
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method to spatially profile the location and distribution of multiple N-linked glycan species in frozen tissues has been extended and improved for the direct analysis of glycans in clinically derived formalin-fixed paraffin-embedded (FFPE) tissues. Formalin-fixed tissues from normal mouse kidney, human pancreatic and prostate cancers, and a human hepatocellular carcinoma tissue microarray were processed by antigen retrieval followed by on-tissue digestion with peptide N-glycosidase F. The released N-glycans were detected by MALDI-IMS analysis, and the structural composition of a subset of glycans could be verified directly by on-tissue collision-induced fragmentation. Other structural assignments were confirmed by off-tissue permethylation analysis combined with multiple database comparisons. Imaging of mouse kidney tissue sections demonstrates specific tissue distributions of major cellular N-linked glycoforms in the cortex and medulla. Differential tissue distribution of N-linked glycoforms was also observed in the other tissue types. The efficacy of using MALDI-IMS glycan profiling to distinguish tumor from non-tumor tissues in a tumor microarray format is also demonstrated. This MALDI-IMS workflow has the potential to be applied to any FFPE tissue block or tissue microarray to enable higher throughput analysis of the global changes in N-glycosylation associated with cancers.
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Details
- Title
- MALDI imaging mass spectrometry profiling of N-glycans in formalin-fixed paraffin embedded clinical tissue blocks and tissue microarrays
- Creators
- Thomas W Powers - Medical University of South CarolinaBenjamin A Neely - Medical University of South CarolinaYuan Shao - Medical University of South CarolinaHuiyuan Tang - Van Andel InstituteDean A Troyer - Eastern Virginia Medical SchoolAnand S Mehta - Drexel UniversityBrian B Haab - Van Andel InstituteRichard R Drake - Medical University of South Carolina
- Publication Details
- PloS one, v 9(9), pp e106255-e106255
- Publisher
- Public LIbrary of Science (PLOS)
- Grant note
- U01CA168896 / NCI NIH HHS R01CA135087 / NCI NIH HHS P30 CA138313 / NCI NIH HHS R01 CA120206 / NCI NIH HHS R01 CA135087 / NCI NIH HHS R21 CA137704 / NCI NIH HHS U01 CA168856 / NCI NIH HHS UL1 TR000062 / NCATS NIH HHS R21CA137704 / NCI NIH HHS UL1 RR029882 / NCRR NIH HHS U01 CA168896 / NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000341257700045
- Scopus ID
- 2-s2.0-84906965809
- Other Identifier
- 991019318939904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods