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MAP kinase phosphorylation is dispensable for cell division, but required for cell growth in Drosophila
Journal article   Open access

MAP kinase phosphorylation is dispensable for cell division, but required for cell growth in Drosophila

Neena Majumdar, Gerardo L Paez, Shivangi M Inamdar, Mitchell D'Rozario and Daniel R Marenda
Fly (Austin, Tex.), v 4(3), pp 204-212
Jul 2010
DOI: https://doi.org/10.4161/fly.4.3.12001
PMID: 20495373
Featured in Collection :   UN Sustainable Development Goals @ Drexel
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https://doi.org/10.4161/fly.4.3.12001View
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Abstract

Protein Kinases - metabolism Phosphorylation Male Wings, Animal - enzymology Compound Eye, Arthropod - enzymology Cell Enlargement Drosophila Proteins - metabolism Drosophila - enzymology Nerve Tissue Proteins - metabolism Animals Wings, Animal - growth & development Eye Proteins - metabolism Cell Division Female Drosophila - growth & development Compound Eye, Arthropod - growth & development Mitogen-Activated Protein Kinases - metabolism
Proper activation of the Ras/MAPK pathway is broadly required during development, and in many cases, signal transduction downstream of the receptor is linear. Thus, different mechanisms exist to properly regulate the large number of specific developmental outputs that are required by the activation of this pathway. Previously, we have reported a regulated cytoplasmic sequestration of phosphorylated MAPK (pMAPK) in developing Drosophila compound eyes and wings "called MAPK Cytoplasmic Hold". In the developing wing, we have shown that cytoplasmic hold promotes the differentiation of wing vein tissue, while pMAPK nuclear translocation regulates growth and division. We had also suggested that the Ras pathway signals for inducing cell growth and cell division split upstream of the nuclear translocation of MAPK itself. Here, we further refine the role of MAPK in Drosophila. We report evidence that suggests, for the first time, that the phosphorylation of MAPK is itself another step in the regulation of cell growth and division in both Drosophila wing and eye cells. We show that inhibition of MAPK phosphorylation, or pMAPK nuclear translocation, is sufficient to block cell growth, but not cell division. These data suggest that non-phosphorylated MAPK is sufficient to induce cell division, but not cell growth, once inside the nucleus of the cell.

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Collaboration types
Domestic collaboration
Web of Science research areas
Biochemistry & Molecular Biology
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