Journal article
MAP kinase subcellular localization controls both pattern and proliferation in the developing Drosophila wing
Development (Cambridge), v 133(1), pp 43-51
Jan 2006
PMID: 16308331
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Mitogen-activated protein kinases (MAPKs) phosphorylate target proteins in both the cytoplasm and nucleus, and a strong correlation exists between the subcellular localization of MAPK and resulting cellular responses. It was thought that MAPK phosphorylation was always followed by rapid nuclear translocation. However, we and others have found that MAPK phosphorylation is not always sufficient for nuclear translocation in vivo. In the developing Drosophila wing, MAPK-mediated signaling is required both for patterning and for cell proliferation, although the mechanism of this differential control is not fully understood. Here, we show that phosphorylated MAPK (pMAPK) is held in the cytoplasm in differentiating larval and pupal wing vein cells, and we show that this cytoplasmic hold is required for vein cell fate. At the same time, we show that MAPK does move into the nucleus of other wing cells where it promotes cell proliferation. We propose a novel Ras pathway bifurcation in Drosophila and our results suggest a mechanism by which MAPK phosphorylation can signal two different cellular outcomes (differentiation versus proliferation) based on the subcellular localization of MAPK.
Metrics
Details
- Title
- MAP kinase subcellular localization controls both pattern and proliferation in the developing Drosophila wing
- Creators
- Daniel R Marenda - Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USAAlysia D VrailasAloma B RodriguesSummer CookMaureen A PowersJames A LorenzenLizabeth A PerkinsKevin Moses
- Publication Details
- Development (Cambridge), v 133(1), pp 43-51
- Publisher
- Company of Biologists; England
- Grant note
- R01 GM61707 / NIGMS NIH HHS P01 HD39942 / NICHD NIH HHS P01 HD039942 / NICHD NIH HHS R01 GM061707 / NIGMS NIH HHS T32 GM008490 / NIGMS NIH HHS 1 F32 GM073608 / NIGMS NIH HHS R01 EY012537 / NEI NIH HHS R01 EY12537 / NEI NIH HHS F32 GM073608-01A1 / NIGMS NIH HHS F32 GM073608 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000234936400005
- Scopus ID
- 2-s2.0-31644444050
- Other Identifier
- 991014877699204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Developmental Biology