Journal article
MC3T3 E1 cell response to mineralized nanofiber shish kebab structures
Journal of biomedical materials research. Part B, Applied biomaterials, v 109(10), pp 1601-1610
Oct 2021
PMID: 33608965
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Block copolymers (BCPs) are of growing interest because of their extensive utility in tissue engineering, particularly in biomimetic approaches where multifunctionality is critical. We synthesized polycaprolactone‐polyacrylic acid (PCL‐b‐PAA) BCP and crystallized it onto PCL nanofibers, making BCP nanofiber shish kebab (BCP NFSK) structures. When mineralized in 2× simulated body fluid, BCP NFSK mimic the structure of mineralized collagen fibrils. We hypothesized that the addition of a calcium phosphate layer of graded roughness on the nano‐structure of the nanofiber shish kebabs would enhance preosteoblast alkaline phosphatase (ALP) activity, which has been shown to be a critical component in bone matrix formation. The objectives in the study were to investigate the effect of mineralization on cell proliferation and ALP activity, and to also investigate the effect of BCP NFSK periodicity, a structural feature describing the distance between PCL‐b‐PAA crystals on the nanofiber core, on cell proliferation, and ALP activity. ALP activity of cells cultured on the mineralized BCP NFSK template was significantly higher than the nonmineralized BCP NFSK templates. Interestingly, no statistical difference was observed in ALP activity when the periodic varied, indicating that surface chemistry seemed to play a larger role than the surface roughness.
Metrics
Details
- Title
- MC3T3 E1 cell response to mineralized nanofiber shish kebab structures
- Creators
- Tony Yu - Drexel UniversityMark Petrovic - Drexel UniversityAria Attia - Drexel UniversityDiego Galindo - Drexel UniversityMark C. Staub - Drexel UniversitySeyong Kim - Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.Christopher Y. Li - Drexel UniversityMichele Marcolongo - Drexel University
- Publication Details
- Journal of biomedical materials research. Part B, Applied biomaterials, v 109(10), pp 1601-1610
- Publisher
- John Wiley & Sons, Inc
- Number of pages
- 10
- Grant note
- National Science Foundation (1507760)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Materials Science and Engineering
- Web of Science ID
- WOS:000619667700001
- Scopus ID
- 2-s2.0-85101072828
- Other Identifier
- 991019168235004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials