Journal article
Macromolecule mediated transport and retention of Escherichia coli O157:H7 in saturated porous media
Water research (Oxford), v 44(4), pp 1082-1093
01 Feb 2010
PMID: 19853881
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The role of extracellular macromolecules on Escherichia coli 0157:147 transport and retention was investigated in saturated porous media. To compare the relative transport and retention of E. coli cells that are macromolecule rich and deficient, macromolecules were partially cleaved using a proteolytic enzyme. Characterization of bacterial cell surfaces, cell aggregation, and experiments in a packed sand column were conducted over a range of ionic strength (IS). The results showed that macromolecule-related interactions contribute to retention of E. coli O157:H7 and are strongly linked to solution IS. Under low IS conditions (IS <= 0.1 mM), partial removal of the macromolecules resulted in a more negative electrophoretic mobility of cells and created more unfavorable conditions for cell-quartz and cell-cell interactions as suggested by Derjaguin-Landau-Verwey-Overbeek (DLVO) interaction energy profiles and cell aggregation kinetics. Consequently, less retention was observed for enzyme treated cells in the corresponding column experiments. in addition, a time-dependent deposition process (i.e., ripening) was observed for untreated cells, but not for treated cells, supporting the fact that the macromolecules enhanced cell-cell interactions. Additional column experiments for untreated cells under favorable conditions (IS >= 1 mM) showed that a significant amount of the cells were reversibly retained in the column, which contradicts predictions of DLVO theory. Furthermore, a non-monotonic cell retention profile was observed under favorable attachment conditions. These observations indicated that the presence of macromolecules hindered irreversible interactions between the cells and the quartz surface. Published by Elsevier Ltd.
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Details
- Title
- Macromolecule mediated transport and retention of Escherichia coli O157:H7 in saturated porous media
- Creators
- Hyunjung N. Kim - Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USASharon L. Walker - University of California, RiversideScott A. Bradford - U.S. Salinity Laboratory
- Publication Details
- Water research (Oxford), v 44(4), pp 1082-1093
- Publisher
- Elsevier
- Number of pages
- 12
- Grant note
- 2006-02541 / USDA CSREES NRI; United States Department of Agriculture (USDA); National Institute of Food and Agriculture
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Engineering
- Web of Science ID
- WOS:000275551300007
- Scopus ID
- 2-s2.0-76749149111
- Other Identifier
- 991021229902304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Environmental
- Environmental Sciences
- Water Resources