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Journal article
Macrophages of diverse phenotypes drive vascularization of engineered tissues
Science advances, v 6(18), pp eaay6391-eaay6391
May 2020
PMID: 32494664
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Macrophages are key contributors to vascularization, but the mechanisms behind their actions are not understood. Here, we show that diverse macrophage phenotypes have distinct effects on endothelial cell behavior, with resulting effects on vascularization of engineered tissues. In Transwell coculture, proinflammatory M1 macrophages caused endothelial cells to up-regulate genes associated with sprouting angiogenesis, whereas prohealing (M2a), proremodeling (M2c), and anti-inflammatory (M2f) macrophages promoted up-regulation of genes associated with pericyte cell differentiation. In 3D tissue-engineered human blood vessel networks in vitro, short-term exposure (1 day) to M1 macrophages increased vessel formation, while long-term exposure (3 days) caused regression. When human tissue-engineered blood vessel networks were implanted into athymic mice, macrophages expressing markers of both M1 and M2 phenotypes wrapped around and bridged adjacent vessels and formed vessel-like structures themselves. Last, depletion of host macrophages inhibited remodeling of engineered vessels, infiltration of host vessels, and anastomosis with host vessels.
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Details
- Title
- Macrophages of diverse phenotypes drive vascularization of engineered tissues
- Creators
- P L Graney - Drexel UniversityS Ben-Shaul - Technion – Israel Institute of TechnologyS Landau - Technion – Israel Institute of TechnologyA Bajpai - Drexel UniversityB Singh - Drexel UniversityJ Eager - Drexel UniversityA Cohen - Drexel UniversityS Levenberg - Technion – Israel Institute of TechnologyK L Spiller - Drexel University
- Publication Details
- Science advances, v 6(18), pp eaay6391-eaay6391
- Publisher
- American Association for the Advancement of Science (AAAS)
- Grant note
- R01 HL130037 / NHLBI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering; School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000531089700009
- Scopus ID
- 2-s2.0-85084637814
- Other Identifier
- 991019168626904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Medicine, Research & Experimental