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Magnetically actuated alginate scaffold: a novel platform for promoting tissue organization and vascularization
Journal article

Magnetically actuated alginate scaffold: a novel platform for promoting tissue organization and vascularization

Yulia Sapir, Emil Ruvinov, Boris Polyak and Smadar Cohen
Methods in molecular biology (Clifton, N.J.), v 1181, pp 83-95
2014
DOI: https://doi.org/10.1007/978-1-4939-1047-2_8
PMID: 25070329

Abstract

Alginates - chemistry Alginates - pharmacology Animals Cattle Cell Culture Techniques - methods Cell Proliferation - drug effects Cell Survival - drug effects Endothelial Cells - cytology Endothelial Cells - drug effects Glucuronic Acid - chemistry Glucuronic Acid - pharmacology Hexuronic Acids - chemistry Hexuronic Acids - pharmacology Magnetic Fields Magnetite Nanoparticles - chemistry Microscopy, Confocal Neovascularization, Physiologic - drug effects Tissue Engineering Tissue Scaffolds - chemistry
Among the greatest hurdles hindering the successful implementation of tissue-engineered cardiac patch as a therapeutic strategy for myocardial repair is the know-how to promote its rapid integration into the host. We previously demonstrated that prevascularization of the engineered cardiac patch improves cardiac repair after myocardial infarction (MI); the mature vessel networks were generated by including affinity-bound angiogenic factors in the patch and its transplantation on the blood vessel-enriched omentum. Here, we describe a novel in vitro strategy to promote the formation of capillary-like networks in cell constructs without supplementing with angiogenic factors. Endothelial cells (ECs) were seeded into macroporous alginate scaffolds impregnated with magnetically responsive nanoparticles (MNPs), and after pre-culture for 24 h under standard conditions the constructs were subjected to an alternating magnetic field of 40 Hz for 7 days. The magnetic stimulation per se promoted EC organization into capillary-like structures with no supplementation of angiogenic factors; in the non-stimulated constructs, the cells formed sheets or aggregates. This chapter describes in detail the preparation method of the MNP-impregnated alginate scaffold, the cultivation setup for the cell construct under magnetic field conditions, and the set of analyses performed to characterize the resultant cell constructs.

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