Journal article
Maintenance of Normal T Lymphocyte Function after Transfection with SV40 Large T
Cellular immunology, v 149(1), pp 65-81
01 Jun 1993
PMID: 8513512
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Human T lymphocytes in culture undergo cellular senescence similar to that observed in fibroblasts. We recently demonstrated that senescence of T lymphocytes can be postponed by transfection with SV40 large T. In the current study, we examine the functional activity of SV40 large T transfected T lymphocytes. Transfected T cells of both young and elderly subjects appear to display normal T cell function: they cease doubling upon removal of IL-2; in the presence of autologous adherent mononuclear cells they respond to mitogen stimulation and produce IL-2 and IFN-γ, during proliferation; and they express both IL-2 and transferrin receptors similar to those observed in mitogen-stimulated nontransfected T cells. In addition, more than 90% of the transfected cells express both the CD4 and "naive" T cell marker, CD45RA. In order to investigate whether the lack of long lived CD8+ cells reflects phenotypic restriction, separated CD4+ and CDS8+ subpopulations were transfected with SV40 large T. All transfections resulted in extended life spans of CD4+, but not CD8+, cells. Therefore, although SV40 large T transfected T lymphocytes maintain normal function, while demonstrating an extended life span, the effect may be restricted to CD4+ cells.
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Details
- Title
- Maintenance of Normal T Lymphocyte Function after Transfection with SV40 Large T
- Creators
- Qin C. Ryan - Drexel UniversityI.Michael Goonewardene - Drexel UniversityDonna M. Murasko - Drexel University
- Publication Details
- Cellular immunology, v 149(1), pp 65-81
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:A1993LJ14600006
- Scopus ID
- 2-s2.0-0027172747
- Other Identifier
- 991020950771504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell Biology
- Immunology