Journal article
Maintenance of human cytomegalovirus genome in human diploid fibroblast cells
Virology (New York, N.Y.), v 130(1), pp 269-271
1983
PMID: 6314650
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Human embryo lung cells pretreated with a combination of human leukocyte interferon and acyclovir were infected with human cytomegalovirus (HCMV) and treated daily for 14 days with the same inhibitor combination. After removal of the inhibitors, the incubation temperature was maintained at either 37 or 40.5°. Incubation of infected cells at 37° after inhibitor removal resulted in progressive virus-specific cytopathology and eventually total destruction of the cell culture. However, HCMV-infected cells incubated at 40.5° after inhibitor removal exhibited little or no virus-induced cytopathology and HCMV remained noninfectious for 8 days. After extended incubation at 40.5°, infectious HCMV was readily detectable even though virus-specific cytopathology was not evident. Reducing the incubation temperature from 40.5 to 37° resulted in stimulation of infectious virus replication and subsequent destruction of the infected cell culture monolayer within 10 days.
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Details
- Title
- Maintenance of human cytomegalovirus genome in human diploid fibroblast cells
- Creators
- Linda J. Bucher - Pennsylvania State UniversityBrian Wigdahl - Pennsylvania State UniversityFred Rapp - Pennsylvania State University
- Publication Details
- Virology (New York, N.Y.), v 130(1), pp 269-271
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1983RM67900028
- Scopus ID
- 2-s2.0-0021067679
- Other Identifier
- 991020110823204721
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- Web of Science research areas
- Virology